Protsenko V A, Bogadel'nikov I V, Krivoshein Iu S, Spitsyn I F, Karpitskiĭ V V
Ukr Biokhim Zh. 1977 Mar-Apr;49(2):113-6.
The method suggested includes incubation of trypsin with native blood serum and subsequent quantitative determination of trypsin, that did not react with inhibitor, by means of the antitryptic precipitating serum. The method is based on the principle of radial immunodiffusion. In native serum that was not subjected to dilution the antitryptic capacity is 210.0 +/- 27.3 microng/ml in people, 238.7 +/- 33.1 microng/ml in rabbits. A distinct dynamics of changes in the antitryptic capacity of blood serum is observed with experimental hypertrypsinemia.
所建议的方法包括将胰蛋白酶与天然血清一起孵育，然后通过抗胰蛋白酶沉淀血清对未与抑制剂反应的胰蛋白酶进行定量测定。该方法基于放射免疫扩散原理。在未稀释的天然血清中，人的抗胰蛋白酶能力为210.0±27.3微克/毫升，兔子为238.7±33.1微克/毫升。在实验性高胰蛋白酶血症中观察到血清抗胰蛋白酶能力有明显的变化动态。
