Robertson D M, Fröysa B
J Endocrinol. 1979 Apr;81(1):153-5. doi: 10.1677/joe.0.0810153.
There was no loss of biological activity in vitro when the 1st International Reference Preparation (IRP) for human pituitary gonadotrophins [FSH and LH/interstitial cell stimulating hormone (ICSH) for bioassay] code no. 69/104 and the 1st IRP for human pituitary LH/ICSH [for immunoassay] code no. 68/40 were stored for 1 year at -70 degrees C in a buffered 0.8% saline solution containing 1% bovine plasma albumin (BPA). However, storage of the 69/104 preparation at -20 degrees C in either 0.1 or 1% BPA, or at -70 degrees C in the presence of 0.1% BPA showed a small but significant decrease (approximately 10%) in activity over the same period. It is, therefore, advantageous to store these reference preparations at -70 degrees C in the presence of 1% BPA.
当人垂体促性腺激素的第一国际参考制剂（IRP）[用于生物测定的促卵泡激素和促黄体生成素/间质细胞刺激素（ICSH）]编号69/104以及人垂体促黄体生成素/ICSH的第一IRP[用于免疫测定]编号68/40在含有1%牛血浆白蛋白（BPA）的0.8%缓冲盐溶液中于-70℃储存1年时，体外生物活性没有损失。然而，69/104制剂在含有0.1%或1% BPA的溶液中于-20℃储存，或在含有0.1% BPA的情况下于-70℃储存，在同一时期活性均出现了微小但显著的下降（约10%）。因此，将这些参考制剂在含有1% BPA的情况下于-70℃储存是有利的。
