Smiałowska M
J Neural Transm. 1979;45(2):139-48. doi: 10.1007/BF01250089.
Using two fluorescence histochemical methods, formaldehyde-induced fluorescence and sucrose-potassiumphosphate-glyoxylic acid fluorescence (SPG), we studied the effect of 5-hydroxytryptamine receptor stimulation by quipazine (2-[-piperazinyl]quinoline maleate) on monoamine fluorescence in the brain stem of rats. It was found that quipazine in a dose of 5 mg/kg i.p., after 60 min, decreased noradrenaline fluorescence intensity in noradrenergic neurons of the subcoeruleus area and diminished th density of catecholamine terminals visualized in the central part of the dorsal raphé nucleus. In the principal locus coeruleus, the intensity of fluorescence in nerve cells was not changed using either method, but with the SPG procedure, diffuse fluorescence outside cell bodies was observed after quipazine. In dorsal raphé neurons, a slight increase in 5-hydroxytryptamine fluorescence intensity was observed. The results obtained indicate that quipazine, apart from its effect on 5-hydroxytryptamine neurons, may also affect certain noradrenergic neurons.
我们运用两种荧光组织化学方法，即甲醛诱导荧光法和蔗糖 - 磷酸钾 - 乙醛酸荧光法（SPG），研究了喹哌嗪（顺丁烯二酸2 - [-哌嗪基]喹啉）刺激5 - 羟色胺受体对大鼠脑干单胺荧光的影响。结果发现，腹腔注射5毫克/千克剂量的喹哌嗪60分钟后，蓝斑下核去甲肾上腺素能神经元中的去甲肾上腺素荧光强度降低，并且在中缝背核中部观察到的儿茶酚胺终末密度减小。在蓝斑主要部位，两种方法均显示神经细胞的荧光强度未发生变化，但采用SPG方法时，喹哌嗪给药后在细胞体外部观察到了弥漫性荧光。在中缝背神经元中，观察到5 - 羟色胺荧光强度略有增加。所得结果表明，喹哌嗪除了对5 - 羟色胺能神经元有作用外，还可能影响某些去甲肾上腺素能神经元。
