Leroy F, Van Hoeck J, Lejeune B
J Reprod Fertil. 1979 May;56(1):187-91. doi: 10.1530/jrf.0.0560187.
After priming with oestradiol, ovariectomized rats were given 6 days of progesterone treatment in which two doses of 50 ng oestradiol were given on Days 3 and 6. This basic treatment allows the oestradiol-induced (1st injection) disappearance of uterine sensitivity to decidual stimuli to occur. Cycloheximide could not mimic oestrogen action in the production of the uterine refractory state. However, a high dose (500 micrograms per animal) of this inhibitor given with the first injection of oestradiol allowed the uterus to remain in a neutral state and to respond to decidual induction after the second dose of oestradiol. By delaying the injection of cycloheximide after the first oestrogen treatment, protein synthesis requisite to the occurrence of uterine refractoriness would not take place within 12 h after the 'nidatory' oestrogen injection.
用雌二醇预处理后，对去卵巢大鼠进行6天的孕酮治疗，在第3天和第6天给予两剂50 ng的雌二醇。这种基本治疗使雌二醇诱导的（第一次注射）子宫对蜕膜刺激的敏感性消失。放线菌酮在子宫不应期的产生中不能模拟雌激素的作用。然而，在第一次注射雌二醇时给予高剂量（每只动物500微克）的这种抑制剂，可使子宫保持中性状态，并在第二次注射雌二醇后对蜕膜诱导产生反应。通过在第一次雌激素治疗后延迟注射放线菌酮，子宫不应期发生所需的蛋白质合成在“着床”雌激素注射后12小时内不会发生。
