Bell S C
J Reprod Fertil. 1979 May;56(1):255-62. doi: 10.1530/jrf.0.0560255.
The synthesis of a soluble protein (referred to as 'decidualization-associated protein', DAP), has been examined in uterine and placental tissues of rats during pregnancy by means of polyacrylamide gel electrophoretic analysis of [3H]leucine-labelled soluble proteins. No synthesis of the protein was detected in non-implantation regions of the uterus. In implantation site tissue, no synthesis was detected on Days 6 or 7 of pregnancy. Only slight synthesis was present in the endometrium on Day 8, but synthesis rose rapidly from Days 9 to 12 in both the endometrium and myometrium although differences in the rates of increase were observed. Synthesis fell from Day 12 to 14 in both tissues. Synthesis by the myometrium was entirely localized in the mesometrial region, which contains the metrial gland. After Day 12, when the endometrium is represented by the chorioallantoic placenta, synthesis was examined in the labyrinthine and the decidua basalis/basal zone placenta tissues. No synthesis of 'DAP' was detected in the labyrinthine placenta from Day 16 of pregnancy. Synthesis observed in the decidua basalis/basal zone placenta fell dramatically from Day 14 to 20. The pattern of synthesis of 'DAP' during pregnancy suggests a role in the establishment of the chorioallantoic placenta and metrial gland in the rat.
通过对[³H]亮氨酸标记的可溶性蛋白质进行聚丙烯酰胺凝胶电泳分析，研究了大鼠孕期子宫和胎盘组织中一种可溶性蛋白质（称为“蜕膜化相关蛋白”，DAP）的合成情况。在子宫的非着床区域未检测到该蛋白质的合成。在着床部位组织中，妊娠第6天或第7天未检测到合成。妊娠第8天，子宫内膜仅有轻微合成，但从第9天到第12天，子宫内膜和子宫肌层的合成均迅速增加，不过增加速率存在差异。两种组织中，合成在第12天到第14天下降。子宫肌层的合成完全局限于包含蜕膜腺的子宫系膜区域。第12天后，当子宫内膜由绒毛尿囊胎盘代表时，在迷路和基底蜕膜/基底区胎盘组织中检测合成情况。妊娠第16天起，在迷路胎盘中未检测到“DAP”的合成。在基底蜕膜/基底区胎盘中观察到的合成从第14天到第20天急剧下降。孕期“DAP”的合成模式表明其在大鼠绒毛尿囊胎盘和蜕膜腺的建立中起作用。
