Bell S C, Reynolds S, Heald P J
J Reprod Fertil. 1977 Mar;49(2):177-81. doi: 10.1530/jrf.0.0490177.
The synthesis of uterine-soluble proteins during early pregnancy in the rat has been examined by means of dual-isotope labelling techniques and subsequent electrophoretic analysis. A protein of similar electrophoretic mobility to the uterine oestrogen-induced protein was observed, and synthesis of this 'presumptive induced protein' was maximal on Day 4 and Day 6 of pregnancy but low on day 5. Pregnancy associated protein synthesis was observed in many regions on polyacrylamide gels, including the beta-lipoprotein, alpha2-macroglobulin, post-transferrin and albumin regions. Synthesis of the post-transferrin species rapidly increased from Day 4 to reach a maximum on Day 6 in the implantation tissue. The temporal pattern of synthesis of post-transferrin protein and and of 'presumptive induced region' suggests involvement in the processes of cell proliferation and decidualization.
利用双同位素标记技术及随后的电泳分析，对大鼠妊娠早期子宫可溶性蛋白的合成进行了研究。观察到一种电泳迁移率与子宫雌激素诱导蛋白相似的蛋白，这种“假定诱导蛋白”的合成在妊娠第4天和第6天达到最大值，而在第5天较低。在聚丙烯酰胺凝胶的许多区域观察到了妊娠相关蛋白的合成，包括β-脂蛋白、α2-巨球蛋白、转铁蛋白后体和白蛋白区域。在植入组织中，转铁蛋白后体的合成从第4天开始迅速增加，在第6天达到最大值。转铁蛋白后体蛋白和“假定诱导区域”的合成时间模式表明其参与了细胞增殖和蜕膜化过程。
