Henderson K M, Moon Y S
J Reprod Fertil. 1979 May;56(1):89-97. doi: 10.1530/jrf.0.0560089.
The relative aromatizing ability of bovine luteinizing granulosa cells and dispersed luteal cells in tissue culture was studied. Luteinization of granulosa cells, as indicated by steadily increasing progesterone production (from 50 to 300 ng/10(5) cells/day over 4--5 days), was accompanied by a dramatic reduction in their capacity to aromatize exogenous androgen; oestradiol-17 beta production falling from 200 to less than 10 ng/10(5) cells/day over 4--5 days. Luteal cells also had only a very limited capacity to aromatize exogenous androgen, maximum oestradiol-17 beta production being less than 600 pg/10(5) cells/day. The loss in aromatizing capacity of granulosa cells during luteinization was also reflected in the relative endogenous steroid content of non-luteinized granulosa cells and luteal tissue, the former containing high levels of oestradiol-17 beta, less than or equal to 28 ng/mg protein, while the latter, although containing substantial amounts of testosterone, less than or equal to 5.7 ng/g tissue, contained very little oestradiol-17 beta, less than or equal to 0.35 ngG TISSUE. These findings suggest that luteinization of bovine granulosa cells and subsequent corpus luteum formation is associated with a loss in androgen aromatase activity.
研究了牛黄体化颗粒细胞和分散的黄体细胞在组织培养中的相对芳香化能力。颗粒细胞的黄体化表现为孕酮产量稳步增加（在4 - 5天内从50 ng/10⁵细胞/天增加到300 ng/10⁵细胞/天），同时其对外源雄激素的芳香化能力急剧下降；雌二醇-17β产量在4 - 5天内从200 ng/10⁵细胞/天降至不足10 ng/10⁵细胞/天。黄体细胞对外源雄激素的芳香化能力也非常有限，最大雌二醇-17β产量低于600 pg/10⁵细胞/天。颗粒细胞在黄体化过程中芳香化能力的丧失也反映在未黄体化颗粒细胞和黄体组织的相对内源性类固醇含量上，前者含有高水平的雌二醇-17β，≤28 ng/mg蛋白质，而后者虽然含有大量睾酮，≤5.7 ng/g组织，但含有很少的雌二醇-17β，≤0.35 ng/g组织。这些发现表明牛颗粒细胞的黄体化以及随后的黄体形成与雄激素芳香化酶活性的丧失有关。
