Chang S Y, Kung C
Science. 1973 Jun 15;180(4091):1197-9. doi: 10.1126/science.180.4091.1197.
"Pawns" are mutants of Paramecium aurelia in which the process of calcium activation during membrane excitation is genetically impaired, with a corresponding loss of avoiding reactions. Mutants are selected that behave normally when grown at 23 degrees C but as pawns at 35 degrees C. The normal excitation can now be disrupted and restored in the same strain at will.
“兵卒”是双小核草履虫的突变体,其中膜兴奋过程中的钙激活过程在基因上受损,相应地失去了回避反应。筛选出的突变体在23摄氏度生长时表现正常,但在35摄氏度时表现为“兵卒”。现在可以在同一菌株中随意破坏和恢复正常的兴奋过程。