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草履虫热敏感品系突变的遗传分析

Genetic Analyses of Heat-Sensitve Pawn Mutants of PARAMECIUM AURELIA.

机构信息

Department of Biological Sciences, University of California Santa Barbara, California 93106.

出版信息

Genetics. 1973 Sep;75(1):49-59. doi: 10.1093/genetics/75.1.49.

DOI:10.1093/genetics/75.1.49
PMID:17248641
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1213001/
Abstract

Heat-sensitive Pawn (ts Pawns) of Paramecium aurelia behaved normally when grown at 23 degrees but failed to avoid strong stimuli at 35 degrees . Four of the five ts Pawn lines tested were found to be allelic at a locus known also to carry temperature-independent Pawn mutations. The fifth ts Pawn line complemented all the conditional and unconditional Pawn mutants of the two known loci. This result, together with the patterns of F(2) segregation from various crosses, suggested the existence of a third Pawn locus. An additive effect of the unlinked ts Pawn genes was observed. These findings and the significance of ts Pawns as experimental material in behavioral and physiological research are discussed.

摘要

温度敏感型帕氏草履虫棋子(ts Pawns)在 23 度下生长时表现正常,但在 35 度时无法避免强烈刺激。在五个测试的 ts Pawn 品系中,有四个在已知携带温度不依赖型棋子突变的基因座上是等位基因。第五个 ts Pawn 品系补充了两个已知基因座上所有条件和无条件的棋子突变体。这一结果,以及来自各种杂交的 F2 分离模式,表明存在第三个棋子基因座。未连锁的 ts Pawn 基因的加性效应得到了观察。这些发现以及 ts Pawns 作为行为和生理研究实验材料的意义将在文中进行讨论。

相似文献

1
Genetic Analyses of Heat-Sensitve Pawn Mutants of PARAMECIUM AURELIA.草履虫热敏感品系突变的遗传分析
Genetics. 1973 Sep;75(1):49-59. doi: 10.1093/genetics/75.1.49.
2
Temperature-sensitive pawns: conditional behavioral mutants of Paramecium aurelia.温度敏感型草履虫:双小核草履虫的条件行为突变体
Science. 1973 Jun 15;180(4091):1197-9. doi: 10.1126/science.180.4091.1197.
3
Nonbehavioral selection for pawns, mutants of Paramecium aurelia with decreased excitability.草履虫(双小核草履虫)兴奋性降低的突变体——无行为能力的品系的非行为选择。
Genetics. 1976 Nov;84(3):453-68. doi: 10.1093/genetics/84.3.453.
4
Mutants with reduced Ca activation in Paramecium aurelia.在双小核草履虫中钙激活降低的突变体。
J Membr Biol. 1976 Aug 26;28(2-3):277-94. doi: 10.1007/BF01869701.
5
Calcium channels in Paramecium aurelia.草履虫中的钙通道。
Prog Clin Biol Res. 1977;15:105-18.
6
Calcium-induced ciliary reversal in the extracted models of "Pawn", a behavioral mutant of Paramecium.在草履虫行为突变体“兵卒”的提取模型中钙诱导的纤毛反转
Science. 1973 Jan 12;179(4069):195-6. doi: 10.1126/science.179.4069.195.
7
Microinjection of cytoplasm as a test of complementation in Paramecium.微注射细胞质作为草履虫互补性的一种测试方法。
J Cell Biol. 1982 Feb;92(2):559-64. doi: 10.1083/jcb.92.2.559.
8
Calcium channel stability measured by gradual loss of excitability in pawn mutants of Paramecium aurelia.通过草履虫 pawn 突变体兴奋性的逐渐丧失来测量钙通道稳定性。
J Exp Biol. 1976 Dec;65(3):725-36. doi: 10.1242/jeb.65.3.725.
9
An extensive behavioural and genetic analysis of the pawn mutants in Paramecium aurelia.对双小核草履虫中pawn突变体的广泛行为和遗传分析。
Genet Res. 1974 Apr;23(2):165-73. doi: 10.1017/s0016672300014786.
10
Membrane excitability: made temperature-dependent by mutations.膜兴奋性:因突变而受温度影响。
Proc Natl Acad Sci U S A. 1974 Jul;71(7):2703-6. doi: 10.1073/pnas.71.7.2703.

引用本文的文献

1
Micromanipulation in Paramecium: From non-mendelian inheritance to the outlook for versatile micromachines.草履虫的微操作:从非孟德尔遗传到多功能微机器的展望。
J Eukaryot Microbiol. 2022 Sep;69(5):e12909. doi: 10.1111/jeu.12909. Epub 2022 May 4.
2
The cloning by complementation of the pawn-A gene in Paramecium.草履虫中pawn - A基因的互补克隆
Genetics. 1998 Jun;149(2):947-57. doi: 10.1093/genetics/149.2.947.
3
Intra- and interspecific complementation of membrane-inexcitable mutants of Paramecium.草履虫膜不可兴奋突变体的种内和种间互补作用
J Cell Biol. 1983 Aug;97(2):378-82. doi: 10.1083/jcb.97.2.378.
4
Membrane excitability: made temperature-dependent by mutations.膜兴奋性:因突变而受温度影响。
Proc Natl Acad Sci U S A. 1974 Jul;71(7):2703-6. doi: 10.1073/pnas.71.7.2703.
5
Nonbehavioral selection for pawns, mutants of Paramecium aurelia with decreased excitability.草履虫(双小核草履虫)兴奋性降低的突变体——无行为能力的品系的非行为选择。
Genetics. 1976 Nov;84(3):453-68. doi: 10.1093/genetics/84.3.453.
6
Mutants with reduced Ca activation in Paramecium aurelia.在双小核草履虫中钙激活降低的突变体。
J Membr Biol. 1976 Aug 26;28(2-3):277-94. doi: 10.1007/BF01869701.
7
A potassium conductance activated by hyperpolarization in paramecium.草履虫中由超极化激活的钾离子电导。
J Membr Biol. 1978 Oct 19;43(2-3):169-85. doi: 10.1007/BF01933477.

本文引用的文献

1
Temperature-sensitive mutations in Drosophila melanogaster. VII. A mutation (para-ts) causing reversible adult paralysis.黑腹果蝇中的温度敏感突变。VII. 一种导致成虫可逆性麻痹的突变(para-ts)
Proc Natl Acad Sci U S A. 1971 May;68(5):890-3. doi: 10.1073/pnas.68.5.890.
2
Bacteriophage assembly.噬菌体组装
Fed Proc. 1968 Sep-Oct;27(5):1160-6.
3
Calcium-induced ciliary reversal in the extracted models of "Pawn", a behavioral mutant of Paramecium.在草履虫行为突变体“兵卒”的提取模型中钙诱导的纤毛反转
Science. 1973 Jan 12;179(4069):195-6. doi: 10.1126/science.179.4069.195.
4
Genetic modification of electric properties in an excitable membrane (paramecium-calcium conductance-electrophysiological measurements-membrane mutant).可兴奋膜电特性的基因改造(草履虫-钙电导-电生理测量-膜突变体)
Proc Natl Acad Sci U S A. 1972 Jan;69(1):93-7. doi: 10.1073/pnas.69.1.93.