Satow Y, Chang S Y, Kung C
Proc Natl Acad Sci U S A. 1974 Jul;71(7):2703-6. doi: 10.1073/pnas.71.7.2703.
Three mutants of Paramecium aurelia with genetic lesions at two unlinked loci lost their ability to generate action potentials when grown at high temperatures. Action potentials found at room temperature were slightly aberrant. Kinetics of phenotypic changes after temperature shifts showed that excitation is not immediately sensitive to temperature change in these mutants. The initiation of an action potential must rely on many different gene products (presumably membrane proteins) which are open to modification by conditional as well as unconditional mutations.
三种在两个不连锁基因座处有遗传损伤的双小核草履虫突变体，在高温下生长时失去了产生动作电位的能力。在室温下发现的动作电位稍有异常。温度变化后表型变化的动力学表明，在这些突变体中，兴奋对温度变化并非立即敏感。动作电位的引发必须依赖于许多不同的基因产物（可能是膜蛋白），这些产物会受到条件突变和非条件突变的修饰。
