Nelson M, Phipps E J, Watson P G, Watts J R, Zwolenski R
J Clin Pathol. 1973 May;26(5):343-50. doi: 10.1136/jcp.26.5.343.
A method is described for preparing purified hepatitis B antigen (HB Ag) viral antigen without density gradient centrifugation. A method for sensitizing human group O red cells with this preparation is given, together with the technical details of an automated passive haemagglutination technique suitable for the mass screening of blood donors for HB virus and anti-HB antibody.
本文描述了一种无需密度梯度离心即可制备纯化乙肝抗原(HB Ag)病毒抗原的方法。给出了用该制剂致敏人O型红细胞的方法,以及适用于大规模筛查献血者乙肝病毒和抗-HB抗体的自动被动血凝技术的技术细节。