Sheldon G, Brazy J, Tuggle B, Crenshaw C, Brumley G
Pediatr Res. 1979 May;13(5 Pt 1):599-602. doi: 10.1203/00006450-197905000-00004.
The lungs of intrauterine 135--136-day-old lambs were lavaged with amnionic fluid, with or without meconium, to determine the effect on lung phosphatidylcholine (PC) concentration, synthesis, and function. No differences were apparent between animals lavaged with amnionic fluid or amnionic fluid with meconium. When lavaged lungs were compared to nonlavaged controls, no detectable differences were observed in histology or the quantity of saturated (SPC) and unsaturated phosphatidylcholine (UPC). However, the lavaged lungs retained a larger fraction of maximal lung volume at 5 cm H2O distending pressure and the incorporation of (32P) orthophosphate into lung PC was significantly reduced. In addition, two lavaged animals who became acidotic (pH less than 7.20) exhibited decreased incorporation of 14C) palmitate into whole lung unsaturated, and saturated phosphatidylcholine.
These data indicate that amnionic fluid can reduce lamb lung de novo synthesis of PC and may contribute to the alterations in lung PC found in neonatal syndromes of respiratory distress. Acidosis may accentuate this effect.
用羊水（有无胎粪）灌洗宫内135 - 136日龄羔羊的肺，以确定其对肺磷脂酰胆碱（PC）浓度、合成及功能的影响。用羊水灌洗的动物与用含胎粪羊水灌洗的动物之间未发现明显差异。将灌洗后的肺与未灌洗的对照相比，在组织学或饱和磷脂酰胆碱（SPC）和不饱和磷脂酰胆碱（UPC）的量方面未观察到可检测到的差异。然而，在5 cm H₂O扩张压下，灌洗后的肺保留了更大比例的最大肺容积，并且肺PC中（³²P）正磷酸盐的掺入显著减少。此外，两只发生酸中毒（pH小于7.20）的灌洗动物，其¹⁴C棕榈酸掺入全肺不饱和及饱和磷脂酰胆碱的量减少。
这些数据表明羊水可减少羔羊肺PC的从头合成，并可能导致新生儿呼吸窘迫综合征中肺PC的改变。酸中毒可能会加重这种效应。
