Das M, Henderson T, Feig S A
Pediatr Res. 1979 May;13(5 Pt 1):632-4. doi: 10.1203/00006450-197905000-00011.
The metabolic capacity of neonatal monocytes was compared to the metabolic capacity of adult monocytes by two entirely different methods: the selective diminution of monocyte contamination of whole mononuclear cells and the isolation of relatively purified populations of monocytes. Monocyte removal from whole mononuclear cells produced a diminution in the pyruvate kinase (PK) activity (from 28.6 +/- 1.1 to 15.6 +/- 1.2 nmoles/min/10(7) cells) and an increase in adenosine triphosphate (ATP) content (from 7.9 +/- 1.0 to 9.5 +/- 0.8 nmoles/10(7) cells) in adult cells. No change in PK activity (from 13.5 +/- 1.3 to 14.0 +/- 1.3) was observed in cord cells, but the ATP content of cord cells was higher after monocytes depletion (from 4.7 +/- 0.5 to 6.2 +- 0.7). The suggestion of metabolic vulnerability was confirmed by metabolic analysis of isolated adult and cord monocytes. The PK activity of adult monocytes was greater than that of cord monocytes (57 +/- 9 and 25 +/- 0.3, respectively) and the ATP content of adult monocytes (5.7 +/- 0.2) was greater than that of cord monocytes (2.3 +/- 0.1). The data confirm prior observations of diminished energy metabolism in neonatal mononucler cells and suggest that the metabolic perturbations may, in part, correlate with functional immaturity of the neonatal monocyte.
选择性减少全单核细胞中的单核细胞污染以及分离相对纯化的单核细胞群体。从全单核细胞中去除单核细胞后，成人细胞中的丙酮酸激酶（PK）活性降低（从28.6±1.1降至15.6±1.2纳摩尔/分钟/10⁷个细胞），三磷酸腺苷（ATP）含量增加（从7.9±1.0升至9.5±0.8纳摩尔/10⁷个细胞）。在脐血单核细胞中未观察到PK活性的变化（从13.5±1.3至14.0±1.3），但单核细胞耗竭后脐血单核细胞的ATP含量更高（从4.7±0.5至6.2±0.7）。对分离的成人和脐血单核细胞的代谢分析证实了代谢易损性的推测。成人单核细胞的PK活性高于脐血单核细胞（分别为57±9和25±0.3），成人单核细胞的ATP含量（5.7±0.2）高于脐血单核细胞（2.3±0.1）。这些数据证实了先前关于新生儿单核细胞能量代谢降低的观察结果，并表明代谢紊乱可能部分与新生儿单核细胞的功能不成熟相关。
