Das M, Klein W, Feig S A
Pediatr Res. 1977 Oct;11(10 Pt 1):1068-72. doi: 10.1203/00006450-197710000-00008.
The mononuclear cells of newborn infants compared to those of normal adults contain diminished activity of pyruvate kinase (PK) (14.9 +/- 1.2 vs. 22.3 +/- 1.3 mumol/10(10) cells/min) and phosphoglycerate kinase (PGK) (195 +/- 10 vs. 415 +/- 19). These deficiencies are accentuated in the cells of premature infants (4.9 +/- 0,5 and 71 +/- 15, respectively) and are accompanied by reduced activity of adenylate kinase (AK) (14.8 +/- 1.9 vs. 33.6 +/- 2.6). The activities of AK and PGK in adult and neonatal mononuclear cells are stimulated by phytohemagglutinin (PHA). Although the PK activity of adult cells is stimulated by PHA, the PK activity of neonatal cells is not. Mononuclear cell glycolysis is stimulated by PHA in both adults and neonates. The mean lactate production of neonatal cells is slightly lower than it is in adult cells (93 vs. 110 mumol/10(10) cells/hr, unstimulated, and 151 vs. 181, stimulated), but the differences are not statistically significant. The ATP content of neonatal cells (3.8 +/- 0.4 mumol/ 10(10) cells) is reduced compared to adult values (6.0 +/- 0.3). Lactate production is directly correlated with PK activity in adult and neonatal mononuclear cells. Neonatal mononuclear cell PK activity increases to adult levels during the first year of life.
与正常成年人相比，新生儿的单核细胞中丙酮酸激酶（PK）活性降低（14.9±1.2对22.3±1.3μmol/10¹⁰细胞/分钟），磷酸甘油酸激酶（PGK）活性降低（195±10对415±19）。这些缺陷在早产儿的细胞中更为明显（分别为4.9±0.5和71±15），并且伴随着腺苷酸激酶（AK）活性降低（14.8±1.9对33.6±2.6）。成人和新生儿单核细胞中AK和PGK的活性受到植物血凝素（PHA）的刺激。虽然成人细胞的PK活性受到PHA的刺激，但新生儿细胞的PK活性不受影响。PHA刺激成人和新生儿的单核细胞糖酵解。新生儿细胞的平均乳酸产量略低于成人细胞（未刺激时为93对110μmol/10¹⁰细胞/小时，刺激后为151对181），但差异无统计学意义。与成人细胞的值（6.0±0.3）相比，新生儿细胞的ATP含量（3.8±0.4μmol/10¹⁰细胞）降低。成人和新生儿单核细胞中乳酸产量与PK活性直接相关。新生儿单核细胞PK活性在生命的第一年内增加到成人水平。
