Lewis L J, Hoak J C, Maca R D, Fry G L
Science. 1973 Aug 3;181(4098):453-4. doi: 10.1126/science.181.4098.453.
Investigative studies dealing with the properties and functions of endothelial cells have been hampered because there has been little or no success in the isolation, growth, and passage of individual cells in large numbers. We have developed a system whereby pure cultures of endothelial cells derived from umbilical veins can be subcultured for at least five serial passages. Many facets of endothelial function and interaction can be evaluated with the use of this new adaptive system of isolation and culture.
由于在大量分离、培养和传代单个细胞方面几乎没有成功，关于内皮细胞特性和功能的研究受到了阻碍。我们开发了一种系统，通过该系统，源自脐静脉的内皮细胞纯培养物可以进行至少五次连续传代培养。利用这种新的分离和培养适应性系统，可以评估内皮功能和相互作用的许多方面。
