猪肾谷氨酰胺酶同工酶在线粒体内的定位。
The intramitochondrial location of the glutaminase isoenzymes of pig kidney.
作者信息
Crompton M, McGivan J D, Chappell J B
出版信息
Biochem J. 1973 Jan;132(1):27-34. doi: 10.1042/bj1320027.
Abstract
- The glutaminase activity of pig kidney is located almost entirely in the cortex. 2. Pig renal cortex contains two glutaminases, one phosphate-dependent and one phosphate-independent. Both isoenzymes are localized exclusively in the mitochondria. 3. After sonication of the mitochondria, the phosphate-dependent isoenzyme is entirely soluble, whereas approximately half the phosphate-independent isoenzyme is associated with the membranes. 4. In intact mitochondria, the activities of both isoenzymes respond to changes in the pH of the intramitochondrial compartment. 5. It is concluded that both glutaminase isoenzymes are situated in the intramitochondrial compartment, and that the phosphate-independent glutaminase may be bound to the inside of the inner mitochondrial membrane.
摘要
- 猪肾的谷氨酰胺酶活性几乎完全位于皮质。2. 猪肾皮质含有两种谷氨酰胺酶，一种依赖磷酸盐，另一种不依赖磷酸盐。两种同工酶都仅定位于线粒体中。3. 线粒体经超声处理后，依赖磷酸盐的同工酶完全可溶，而约一半不依赖磷酸盐的同工酶与膜相关。4. 在完整的线粒体中，两种同工酶的活性均对线粒体内腔pH值的变化作出反应。5. 得出的结论是，两种谷氨酰胺酶同工酶均位于线粒体内腔，且不依赖磷酸盐的谷氨酰胺酶可能与线粒体内膜内侧结合。
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