运用分析等速电泳法对贫血患者尿液中的糖蛋白 - 蛋白质混合物进行研究。

Investigation of a glycoprotein-protein mixture from urine of anemic patients by analytical isotachophoresis.

作者信息

Thorn W, Weiland E, Wasmus G

出版信息

Res Exp Med (Berl). 1979 May 30;175(2):155-8. doi: 10.1007/BF01851822.

Abstract

Nine glycoprotein-protein fractions prepared of erythropoietin-active crude urinary protein and separated by gel filtration on Sephadex G-50, ion exchange chromatography on Sephadex DEAE A-50, gel filtration on Sephacryl S-200 and Sepharose 6B were investigated by analytical isotachophoresis. 7 to 38 components could be separated of every chromatographic fraction investigated using chloride as leading and glycinate as terminating electrolyte. Improvement of resolution is possible by variation of buffer system conditions.

摘要

对从具有促红细胞生成素活性的粗制尿蛋白中制备的9种糖蛋白-蛋白质级分进行了研究，这些级分通过在Sephadex G-50上进行凝胶过滤、在Sephadex DEAE A-50上进行离子交换色谱、在Sephacryl S-200和Sepharose 6B上进行凝胶过滤来分离，采用分析等速电泳法进行研究。以氯化物作为先导电解质、甘氨酸盐作为终止电解质，对所研究的每个色谱级分可分离出7至38个组分。通过改变缓冲系统条件有可能提高分离度。