采用制备型等速电泳法对人尿促红细胞生成素进行部分纯化的程序。

Partial purification procedure for human urinary erythropoietin by preparative isotachophoresis.

作者信息

Puschmann M, Thorn W, Yen Y

出版信息

Res Exp Med (Berl). 1978 Sep 25;173(3):293-6. doi: 10.1007/BF01851501.

Abstract

Human urinary erythropoietic-active crude protein was isotachophoretic fractionated in a LKB Uniphor apparatus equipped with a plastic column using polyacrylamide as a stabilizing medium, phosphat ions as leading and glycinat ions as terminating electrolytes and Ampholine carrier ampholytes (pH: 5--7) as spacers. The exhypoxic polycythemic mouse method to assay erythropoietic activity showed that the majority of the hormon was sharply resolved. Under these conditions preparative isotachophoresis demonstrated a higher purification factor compared to other biochemical preparation-methods.

摘要

人尿促红细胞生成活性粗蛋白在配备塑料柱的LKB Uniphor装置中进行等速电泳分级分离，使用聚丙烯酰胺作为稳定介质，磷酸盐离子作为先导电解质，甘氨酸离子作为终止电解质，两性电解质载体两性电解质（pH：5-7）作为间隔物。用低氧性红细胞增多症小鼠法测定促红细胞生成活性表明，大部分激素得到了清晰的分离。在这些条件下，制备性等速电泳显示出比其他生化制备方法更高的纯化因子。