尿酸。其与人类血清白蛋白相互作用的表征。
Uric acid. Characterization of its interaction with human serum albumin.
作者信息
Campion D S, Bluestone R, Klinenberg J R
出版信息
J Clin Invest. 1973 Sep;52(9):2383-7. doi: 10.1172/JCI107427.
Abstract
Binding of sodium urate to human serum albumin (HSA) was measured by continuous ultrafiltration at pH 7.4 and ionic strength 0.16 over the concentration range 1-13 mg/100 ml. The percent sodium urate bound to 5 g/100 ml HSA was constant over this concentration range: 30.3 (SE+/-0.6)% being bound at 4 degrees C, 22.6+/-0.3% at 22.5 degrees C, and 19.6+/-0.3% at 37 degrees C. Derived association constants, assuming one binding site were 6.0 x 10(2) M(-1) (4 degrees C), 4.47 x 10(2) M(-1) (22.5 degrees C), and 3.88 x 10(2) M(-1) (37 degrees C).
摘要
在pH 7.4和离子强度0.16条件下，通过连续超滤法测定了尿酸钠在1-13mg/100ml浓度范围内与人类血清白蛋白（HSA）的结合情况。在此浓度范围内，与5g/100ml HSA结合的尿酸钠百分比保持恒定：4℃时为30.3（标准误±0.6）%，22.5℃时为22.6±0.3%，37℃时为19.6±0.3%。假设存在一个结合位点，推导出的缔合常数分别为6.0×10²M⁻¹（4℃）、4.47×10²M⁻¹（22.5℃）和3.88×10²M⁻¹（37℃）。
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Scand J Clin Lab Invest. 1965;17(5):467-75.
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