Elovaara E, Hemminki K, Vainio H
Toxicology. 1979 Feb;12(2):111-9. doi: 10.1016/0300-483x(79)90037-4.
Toluene and 5 aliphatic chlorinated hydrocarbons of wide industrial use were injected into the air space of fertilized chicken eggs at 2, 3 and 6 days of incubation. The embryotoxicity was evaluated as survival and death incidences after 14 days of incubation, and also the weights and lengths of the embryos were recorded. The approximate LD50 value for trichloroethylene and trichloroethanes varied between 50 and 100 mumol/egg while for toluene, tetrachloroethylene and methylene chloride it was over 100 mumol/egg. Macroscopic malformations of various kinds were produced with doses of 5-100 mumol/egg. The teratogenic potential of the tested compounds decreased in the following order: 1,1,1-trichloroethane greater than trichloroethylene greater than methylene chloride, tetrachloroethylene, 1,1,2-trichloroethane greater than toluene greater than olive oil control.
将甲苯和5种工业上广泛使用的脂肪族氯代烃注入孵化2天、3天和6天的受精鸡蛋的气室中。在孵化14天后，根据存活和死亡发生率评估胚胎毒性，并记录胚胎的重量和长度。三氯乙烯和三氯乙烷的近似半数致死剂量（LD50）值在50至100微摩尔/蛋之间，而甲苯、四氯乙烯和二氯甲烷的该值超过100微摩尔/蛋。剂量为5 - 100微摩尔/蛋时会产生各种宏观畸形。受试化合物的致畸潜力按以下顺序降低：1,1,1 - 三氯乙烷＞三氯乙烯＞二氯甲烷、四氯乙烯、1,1,2 - 三氯乙烷＞甲苯＞橄榄油对照。
