通过放射免疫测定法简化尿醛固酮测量
Simplification of urinary aldosterone measurement by radioimmunoassay.
作者信息
Cope C L, Loizou S
出版信息
J Clin Pathol. 1973 Aug;26(8):628-34. doi: 10.1136/jcp.26.8.628.
Abstract
An anti-aldosterone serum has been used to measure urinary aldosterone concentration. A single stage of paper chromatography provides an extract of sufficient purity. Further purification by additional chromatography in a different solvent system only improves the purity by 3% +/- 8 (SD). Blanks are negligible and recovery of added aldosterone is 104% +/- 10 (SD).A simplified rapid screening assay capable of revealing gross deviations from the normal is also suggested. The diagnostic value of the assay is indicated.
摘要
一种抗醛固酮血清已被用于测量尿醛固酮浓度。单级纸色谱法可提供纯度足够的提取物。在不同溶剂系统中通过额外的色谱法进一步纯化，纯度仅提高3%±8（标准差）。空白值可忽略不计，添加醛固酮的回收率为104%±10（标准差）。还提出了一种能够揭示与正常情况的重大偏差的简化快速筛查测定法。该测定法的诊断价值也已表明。
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