Karpenko V I, Ianishpol'skiĭ V V, Tertykh V A
Ukr Biokhim Zh (1978). 1979 Jul-Aug;51(4):387-92.
The article deals with conditions for immobilization of methane-oxidizing bacteria cells as well as with catalytic properties of the immobilized cells. The method of immobilization in polyacrylamide gel is shown to be not suitable for cells of methane-oxidizing bacteria. The greatest number of cells (85%) is immobilized on sylochrome modified by cyanuric chloride. However, the catalytic properties of the methane-oxidizing bacteria are better retained when the bacteria are immobilized on sylochrome modified by isocyanate. A stand installation is created for studying the catalytic properties of the immobilized cells oxidizing substrates in the gas phase.
本文探讨了甲烷氧化细菌细胞固定化的条件以及固定化细胞的催化特性。结果表明，聚丙烯酰胺凝胶固定化方法不适用于甲烷氧化细菌细胞。最多数量的细胞（85%）固定在经三聚氯氰改性的硅胶上。然而，当细菌固定在经异氰酸酯改性的硅胶上时，甲烷氧化细菌的催化特性能得到更好的保留。搭建了一个试验装置，用于研究固定化细胞在气相中氧化底物的催化特性。
