Myers D M, Varela-Díaz V M, Siniuk A A
Appl Microbiol. 1973 Apr;25(4):514-6. doi: 10.1128/am.25.4.514-516.1973.
A comparison was made of the survival of 28 leptospiral serotypes in Fletcher semisolid medium and in the same medium containing a basal layer of Fletcher medium plus 0.7% of agar and 0.5% of activated animal charcoal. A year after culture, more motile leptospires were observed by microscope examination in the biphasic medium. Two years after culture, 4 serotypes grown in the biphasic medium and 11 in Fletcher medium did not show motile cells. Nineteen of the serotypes maintained in Fletcher medium and 25 in the biphasic medium for 2 years grew on subculture into Fletcher medium. Subcultures from the biphasic medium showed the characteristic leptospiral ring growth earlier during the incubation period.
对28种钩端螺旋体血清型在弗莱彻半固体培养基以及含有弗莱彻培养基底层加0.7%琼脂和0.5%活性动物炭的相同培养基中的存活情况进行了比较。培养一年后，通过显微镜检查在双相培养基中观察到更多活动的钩端螺旋体。培养两年后，在双相培养基中生长的4种血清型和在弗莱彻培养基中生长的11种血清型未显示出活动细胞。在弗莱彻培养基中保存两年的19种血清型和在双相培养基中保存两年的25种血清型在转接到弗莱彻培养基中时能够生长。来自双相培养基的传代培养物在潜伏期早期显示出典型的钩端螺旋体环状生长。
