感受态流感嗜血杆菌脱氧核糖核酸中的单链区域。
Single-strand regions in the deoxyribonucleic acid of competent Haemophilus influenzae.
作者信息
LeClerc J E, Setlow J K
出版信息
J Bacteriol. 1975 Jun;122(3):1091-102. doi: 10.1128/jb.122.3.1091-1102.1975.
Abstract
The deoxyribonucleic acid (DNA) of competent wild-type Haemophilus influenzae and rec1 mutant cells contains single-strand regions, as judged by alkaline sucrose sedimentation, benzoylated naphthoylated diethylaminoethyl-cellulose fractionation, and digestion with an enzyme specific for single-strand regions in DNA. In contrast, the DNA of competent rec2 cells does not contain single-strand regions. Since transforming DNA does not associate with recipient DNA in the rec2 mutant as it does in wild type and rec1, it is concluded that the single-strand regions in the DNA of the competent cells are important for an early step in recombination between cell DNA and transforming DNA.
摘要
通过碱性蔗糖沉降、苯甲酰化萘甲酰化二乙氨基乙基纤维素分级分离以及用一种对DNA单链区域具有特异性的酶进行消化判断，感受态野生型流感嗜血杆菌和rec1突变体细胞的脱氧核糖核酸（DNA）含有单链区域。相比之下，感受态rec2细胞的DNA不含有单链区域。由于在rec2突变体中，转化DNA不像在野生型和rec1中那样与受体DNA结合，因此得出结论，感受态细胞DNA中的单链区域对于细胞DNA与转化DNA之间重组的早期步骤很重要。
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