霍乱毒素中皮肤通透性活性的选择性破坏：酸对霍乱肠毒素的影响。
Selective destruction of skin permeability activity in cholera toxin: effect of acid on cholera enterotoxin.
作者信息
Bhattacharjee A K, Mosley W H
出版信息
Infect Immun. 1973 Aug;8(2):133-6. doi: 10.1128/iai.8.2.133-136.1973.
Abstract
The effect of treatment of purified cholera enterotoxin with acid at pH 2.0 for 1 h followed by neutralization of the acid on the biological activity of the toxin has been studied. It was found that the material loses 85 to 96% of its skin permeability activity and this loss is neither reversed nor increased after 28 days of storage at 5 to 10 C. Other biological properties such as diarrheagenic activity, lipolysis-stimulating activity, and the lethal dose for mice remain unaffected by acid treatment. These observations indicate that the skin permeability activity is more sensitive to relatively minor changes in molecular structure than are the diarrheal, lipolytic, and mouse lethality activities.
摘要
研究了将纯化的霍乱肠毒素在pH 2.0的酸性条件下处理1小时，然后中和酸，这对毒素生物活性的影响。结果发现，该物质失去了85%至96%的皮肤渗透活性，并且在5至10摄氏度下储存28天后，这种活性损失既没有逆转也没有增加。其他生物学特性，如致腹泻活性、刺激脂肪分解活性和对小鼠的致死剂量，均不受酸处理的影响。这些观察结果表明，与腹泻、脂肪分解和小鼠致死活性相比，皮肤渗透活性对分子结构中相对较小的变化更为敏感。
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