Coulson A S, Cohn R
Immunology. 1973 Aug;25(2):307-16.
Normal human blood lymphocytes were cultured in chicken plasma clots with a series of PHA-P dilutions. In this situation direct cell-to-cell contact was prevented, and thus a morphological estimate of the actual number of PHA responsive cells was possible in the absence of any cell-to-cell co-operation which might be mediated via cell contact. The distorting effects of transformed cell proliferation on quantification, and of death of the original small lymphocyte population, were similarly obviated. Between 9 and 20 per cent of those lymphocytes separated by methylcellulose and iron were PHA responsive at the optimal dose of PHA-P.
将正常人血液淋巴细胞与一系列稀释的植物血凝素-P（PHA-P）在鸡血浆凝块中培养。在这种情况下，细胞间的直接接触被阻断，因此，在不存在任何可能通过细胞接触介导的细胞间合作的情况下，可以对PHA反应性细胞的实际数量进行形态学评估。同样避免了转化细胞增殖对定量的干扰以及原始小淋巴细胞群体死亡的影响。在PHA-P的最佳剂量下，经甲基纤维素和铁分离的淋巴细胞中有9%至20%对PHA有反应。
