淋巴细胞对植物血凝素转化的标准化
Standardization of lymphocyte transformation to phytohaemagglutinin.
作者信息
Yamamura M
出版信息
Clin Exp Immunol. 1973 Jul;14(3):457-67.
Abstract
In contrast to the million cells used by many current workers, careful analysis of lymphocyte transformation to phytohaemagglutinin determined optimum conditions as (i) 200,000 lymphocytes in 1 ml of culture fluid in 100 × 12-mm round-bottomed glass tubes (ii) phytohaemagglutinin at 1 μg/tube (iii) H-thymidine at 1 μCi/tube (iv) with a convenient incorporation time 4 hr (v) starting 44 hr after adding phytohaemagglutinin. It was then found 10% autologous serum was the optimum concentration in the culture fluid. The log–normal range of transformation indices for young adults was 27–93–255.
摘要
与许多当前研究人员使用的数百万个细胞不同，对淋巴细胞向植物血凝素转化的仔细分析确定了最佳条件为：（i）在100×12毫米圆底玻璃管中的1毫升培养液中加入200,000个淋巴细胞；（ii）每管加入1微克植物血凝素；（iii）每管加入1微居里的H-胸腺嘧啶核苷；（iv）合适的掺入时间为4小时；（v）在加入植物血凝素后44小时开始。随后发现，培养液中10%的自体血清是最佳浓度。年轻人转化指数的对数正态范围为27 - 93 - 255。
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