Presant C A, Parker S
Clin Exp Immunol. 1977 Jan;27(1):183-9.
Calf sub-mandibular lymph node lymphocytes and human peripheral blood lymphocytes were oxidized by NaIO4. In liquid cell cultures, both types of cells were transformed with enhanced DNA synthesis. In mixed lymphocyte cultures, untreated human lymphocytes were transformed by irradiated-oxidized human cells. However, untreated calf lymphocytes were not transformed by irradiated-oxidized calf cells. Either human or calf lymphocytes were isolated from cell-cell interaction in agarose on slides. After 2-3 days of culture, cells were pulsed with tritiated thymidine. Autoradiography indicated greater numbers of cells with active DNA synthesis in NaIO4-oxidized calf cells and in calf cells incubated with Phaseolus vulgaris E-PHA compared to untreated lymphocytes. Similarly treated human cells did not show any increase in the numbers of cells with active DNA synthesis. No enhancement of DNA synthesis was seen in oxidized calf cells which were reduced with NaBH4 or cultured with hydroxyurea. In these restricted in vitro systems, calf cells are 'transformed' independent of cell-cell interaction, while human cells are not.
用高碘酸钠氧化小牛下颌下淋巴结淋巴细胞和人外周血淋巴细胞。在液体细胞培养中，两种类型的细胞均发生转化，DNA合成增强。在混合淋巴细胞培养中，未处理的人淋巴细胞被经辐射氧化的人细胞转化。然而，未处理的小牛淋巴细胞未被经辐射氧化的小牛细胞转化。人或小牛淋巴细胞均从载玻片上琼脂糖中的细胞 - 细胞相互作用中分离出来。培养2 - 3天后，用氚标记的胸腺嘧啶核苷脉冲处理细胞。放射自显影显示，与未处理的淋巴细胞相比，在经高碘酸钠氧化的小牛细胞以及与菜豆E - PHA孵育的小牛细胞中，有更多具有活跃DNA合成的细胞。经类似处理的人细胞中，具有活跃DNA合成的细胞数量未显示出任何增加。在用硼氢化钠还原或用羟基脲培养的氧化小牛细胞中，未观察到DNA合成增强。在这些受限的体外系统中，小牛细胞“转化”独立于细胞 - 细胞相互作用，而人细胞则不然。
