多粘芽孢杆菌天然的、锌制备的和锰制备的金属蛋白酶的物理化学性质
Physiochemical properties of the native, zinc- and manganese-prepared metalloprotease of Bacillus polymyxa.
作者信息
Griffin P J, Fogarty W M
出版信息
Appl Microbiol. 1973 Aug;26(2):191-5. doi: 10.1128/am.26.2.191-195.1973.
Abstract
The neutral protease of Bacillus polymyxa had a broad pH optimum (6.0 to 7.2) for activity at 37 C. The enzyme was most stable at pH 5.6 to 5.8. The protease had an optimum temperature of 37 C and was quite thermostable up to 35 C, but at higher temperatures the stability decreased rapidly. The substrate specificity of the protease was similar to that of the neutral proteases of other members of the genus Bacillus. The enzyme was shown to be a zinc metalloprotease. However, manganous ions had a greater activating and stabilizing influence on the activity of this enzyme than zinc. Replacement of zinc in the native enzyme by manganese resulted in a 50% increase in activity. In addition, the prepared manganese metalloprotease had higher temperature and more alkaline pH optima than the native enzyme.
摘要
多粘芽孢杆菌的中性蛋白酶在37℃时活性的最适pH范围较宽（6.0至7.2）。该酶在pH 5.6至5.8时最稳定。该蛋白酶的最适温度为37℃，在高达35℃时相当耐热，但在较高温度下稳定性迅速下降。该蛋白酶的底物特异性与芽孢杆菌属其他成员的中性蛋白酶相似。该酶被证明是一种锌金属蛋白酶。然而，锰离子对该酶活性的激活和稳定作用比锌更大。用锰替代天然酶中的锌导致活性增加50%。此外，制备的锰金属蛋白酶比天然酶具有更高的温度和更碱性的最适pH。
相似文献
1
Physiochemical properties of the native, zinc- and manganese-prepared metalloprotease of Bacillus polymyxa.多粘芽孢杆菌天然的、锌制备的和锰制备的金属蛋白酶的物理化学性质
Appl Microbiol. 1973 Aug;26(2):191-5. doi: 10.1128/am.26.2.191-195.1973.
2
Isolation and partial characterization of a thermostable extracellular protease of Bacillus polymyxa B-17.多粘芽孢杆菌B-17耐热胞外蛋白酶的分离及部分特性研究
Int J Food Microbiol. 1998 Jul 21;42(3):139-45. doi: 10.1016/s0168-1605(98)00061-0.
3
Production and purification of the metalloprotease of Bacillus polymyxa.多粘芽孢杆菌金属蛋白酶的生产与纯化
Appl Microbiol. 1973 Aug;26(2):185-90. doi: 10.1128/am.26.2.185-190.1973.
4
Proteases of the genus Bacillus. I. Neutral proteases.芽孢杆菌属蛋白酶。I. 中性蛋白酶。
Biotechnol Bioeng. 1970 Mar;12(2):179-212. doi: 10.1002/bit.260120205.
5
Influence of growth conditions on the production of extracellular proteolytic enzymes in Paenibacillus peoriae NRRL BD-62 and Paenibacillus polymyxa SCE2.生长条件对皮奥里亚类芽孢杆菌NRRL BD - 62和多粘类芽孢杆菌SCE2胞外蛋白水解酶产生的影响
Lett Appl Microbiol. 2006 Dec;43(6):625-30. doi: 10.1111/j.1472-765X.2006.02015.x.
6
Novel alkaline- and heat-stable serine proteases from alkalophilic Bacillus sp. strain GX6638.来自嗜碱芽孢杆菌GX6638菌株的新型碱性和热稳定丝氨酸蛋白酶。
J Bacteriol. 1987 Jun;169(6):2762-8. doi: 10.1128/jb.169.6.2762-2768.1987.
7
Purification and some properties of the extracellular protease of Bacillus pumilus.短小芽孢杆菌胞外蛋白酶的纯化及某些性质
Folia Microbiol (Praha). 1970;15(3):169-75. doi: 10.1007/BF02873080.
8
Some properties of two purified fibinolytic enzymes from Bacillus subtilis and B. polymyxa.
Z Allg Mikrobiol. 1980;20(6):383-7. doi: 10.1002/jobm.3630200604.
9
Characterization of solvent stable extracellular protease from Bacillus koreensis (BK-P21A).从韩国芽孢杆菌（BK-P21A）中提取具有溶剂稳定性的胞外蛋白酶的特性研究。
Int J Biol Macromol. 2013 May;56:162-8. doi: 10.1016/j.ijbiomac.2013.02.014. Epub 2013 Feb 26.
10
Purification and characterization of Vibrio vulnificus protease.创伤弧菌蛋白酶的纯化与特性分析
Microbiol Immunol. 1987;31(1):13-25. doi: 10.1111/j.1348-0421.1987.tb03064.x.
引用本文的文献
1
Mechanical versus enzymatic isolation of stromal vascular fraction cells from adipose tissue.从脂肪组织中机械分离与酶解分离基质血管成分细胞
Springerplus. 2015 Nov 23;4:713. doi: 10.1186/s40064-015-1509-2. eCollection 2015.
2
Structure of gentlyase, the neutral metalloprotease of Paenibacillus polymyxa.多粘芽孢杆菌的中性金属蛋白酶温和酶的结构
Acta Crystallogr D Biol Crystallogr. 2013 Jan;69(Pt 1):24-31. doi: 10.1107/S0907444912041169. Epub 2012 Dec 20.
3
Tissue dissociation enzymes for isolating human islets for transplantation: factors to consider in setting enzyme acceptance criteria.用于分离用于移植的人胰岛的组织解离酶：设置酶接受标准时需要考虑的因素。
Transplantation. 2011 Jan 27;91(2):137-45. doi: 10.1097/TP.0b013e3181ffff7d.
4
Tissue dissociation enzyme neutral protease assessment.组织解离酶中性蛋白酶评估。
Transplant Proc. 2010 Jul-Aug;42(6):2052-4. doi: 10.1016/j.transproceed.2010.05.118.
5
On the origin of life in the zinc world. 2. Validation of the hypothesis on the photosynthesizing zinc sulfide edifices as cradles of life on Earth.论锌世界中的生命起源。2. 关于硫化锌结构体作为地球生命摇篮的光合作用假说的验证。
Biol Direct. 2009 Aug 24;4:27. doi: 10.1186/1745-6150-4-27.
6
Development of an owoh-type product from African yam beans (Sphenostylis stenocarpa) (Hoechst (ex. A. Rich.) Harms.) seeds by solid substrate fermentation.
Plant Foods Hum Nutr. 2001;56(2):183-94. doi: 10.1023/a:1011185513717.
7
Production and purification of the metalloprotease of Bacillus polymyxa.多粘芽孢杆菌金属蛋白酶的生产与纯化
Appl Microbiol. 1973 Aug;26(2):185-90. doi: 10.1128/am.26.2.185-190.1973.
8
Extracellular enzyme synthesis in the genus Bacillus.芽孢杆菌属中的胞外酶合成
Bacteriol Rev. 1977 Sep;41(3):711-53. doi: 10.1128/br.41.3.711-753.1977.
9
Metalloprotease from Bacillus thuringiensis.来自苏云金芽孢杆菌的金属蛋白酶。
Appl Microbiol. 1975 Sep;30(3):354-61. doi: 10.1128/am.30.3.354-361.1975.
10
The extracellular metalloprotease of Serratia marcescens: I. Purification and characterization.粘质沙雷氏菌的细胞外金属蛋白酶：I. 纯化与特性分析。
Mol Cell Biochem. 1976 Nov 30;13(2):95-100. doi: 10.1007/BF01837059.
本文引用的文献
1
BACILLUS SUBTILIS NEUTRAL PROTEINASE. I. A ZINC ENZYME OF HIGH SPECIFIC ACTIVITY.枯草芽孢杆菌中性蛋白酶。I. 一种高比活性的锌酶。
J Biol Chem. 1964 Nov;239:3706-15.
2
Purification and properties of an alpha-amylase from facultative thermophilic bacteria.兼性嗜热菌α-淀粉酶的纯化及性质
Arch Biochem Biophys. 1955 Jan;54(1):154-61. doi: 10.1016/0003-9861(55)90018-7.
3
The presence of lysinal (2,6-diaminohexanal) in tropocollagen.原胶原蛋白中赖氨醛（2,6 - 二氨基己醛）的存在。
Biochem Biophys Res Commun. 1967 Jun 9;27(5):546-51. doi: 10.1016/s0006-291x(67)80022-6.
4
Physical chemical studies on the age changes in rat tail tendon collagen.大鼠尾腱胶原蛋白年龄变化的物理化学研究
Biochim Biophys Acta. 1968 Jan 22;154(1):208-19. doi: 10.1016/0005-2795(68)90273-0.
5
Biochemistry of collagen crosslinking. Isolation of a new crosslink, hydroxylysinohydroxynorleucine, and its reduced precursor, dihydroxynorleucine, from bovine tendon.胶原蛋白交联的生物化学。从牛腱中分离出一种新的交联物——羟赖氨酰羟正亮氨酸及其还原前体二羟正亮氨酸。
Biochem Biophys Res Commun. 1970 Dec 24;41(6):1597-604. doi: 10.1016/0006-291x(70)90571-1.
6
Proteases of the genus Bacillus. I. Neutral proteases.芽孢杆菌属蛋白酶。I. 中性蛋白酶。
Biotechnol Bioeng. 1970 Mar;12(2):179-212. doi: 10.1002/bit.260120205.
7
Some properties of a protease from Bacillus polymyxa.多粘芽孢杆菌蛋白酶的一些特性
Biochem J. 1971 Dec;125(4):109P. doi: 10.1042/bj1250109pa.
8
Bacillus cereus neutral protease.蜡样芽孢杆菌中性蛋白酶。
Biochim Biophys Acta. 1971 Oct;251(1):74-8. doi: 10.1016/0005-2795(71)90061-4.
9
Bacillus megaterium neutral protease, a zinc-containing metalloenzyme.巨大芽孢杆菌中性蛋白酶，一种含锌金属酶。
Biochim Biophys Acta. 1971 Mar 23;229(3):829-35. doi: 10.1016/0005-2795(71)90302-3.
10
Biochemical and physiological properties of an extracellular protease produced by Bacillus megaterium.巨大芽孢杆菌产生的一种胞外蛋白酶的生化和生理特性
Biotechnol Bioeng. 1969 Nov;11(6):1233-46. doi: 10.1002/bit.260110617.
Zotero 插件