Thierry A M, Blanc G, Sobel A, Stinus L, Glowinski J
Science. 1973 Nov 2;182(4111):499-501. doi: 10.1126/science.182.4111.499.
The destruction of ascending noradreniergic pathways by bilateral microinjections of 6-hydroxydopamnine made laterally to the pedunculus cerebellaris superior completely abolished the in vitro synthesis of [(3)H]norepinephrine from L-[(3)H]tyrosine in slices and in synaptosomes of the rat cortex. However, normal [(3)H]dopamine synthesis could still be observed in both cortical preparations from animals with lesions. These results provide the first biochemical support for the existence of dopaminergic terminals independent of noradrenergic terminals in the rat cortex.
通过向大鼠小脑上脚外侧双侧微量注射6-羟基多巴胺来破坏上行去甲肾上腺素能通路，完全消除了大鼠皮质切片和突触体中由L-[(3)H]酪氨酸体外合成[(3)H]去甲肾上腺素的过程。然而，在有损伤的动物的两种皮质制剂中仍可观察到正常的[(3)H]多巴胺合成。这些结果为大鼠皮质中存在独立于去甲肾上腺素能终末的多巴胺能终末提供了首个生化支持。
