Brosious E M, Schmidt R M
Am J Clin Pathol. 1979 Sep;72(3):421-5. doi: 10.1093/ajcp/72.3.421.
Samples of whole blood from four hematologically normal adults and from two individuals with increased fetal hemoglobin levels were shipped to laboratories participating in the 1976 and 1977 Center for Disease Control (CDC) hemoglobinopathy proficiency testing surveys. The data from these surveys were used to evaluate the interlaboratory variability of current methods used to quantitate hemoglobin F (Hb F). Results of Hb F quantitation obtained from more than 100 laboratories than voluntarily participated in the survey were compared with those obtained from 21 reference laboratories. Individual values for all samples varied greatly among laboratories and among methods. Results returned by most of the laboratories were outside two standard deviations of the reference laboratory mean and were not accurate enough to differentiate between a normal level and an increased, abnormal level.
从四名血液学正常的成年人以及两名胎儿血红蛋白水平升高的个体采集的全血样本，被运送到参与1976年和1977年疾病控制中心（CDC）血红蛋白病能力验证调查的实验室。这些调查的数据用于评估当前用于定量血红蛋白F（Hb F）的方法在实验室间的变异性。将来自100多个自愿参与调查的实验室获得的Hb F定量结果与来自21个参考实验室的结果进行比较。所有样本的个体值在不同实验室和不同方法之间差异很大。大多数实验室返回的结果超出了参考实验室均值的两个标准差，准确性不足以区分正常水平和升高的异常水平。
