Strunk R C, Payne C M, Nagle R B, Kunke K
Am J Pathol. 1979 Sep;96(3):753-70.
Studies in humans who have received Intralipid (IL) have demonstrated the presence of a fat pigment and fat droplets in reticuloendothelial phagocytic cells. Clinical data and in vitro studies suggest that these cells do not function normally. We have studied the effect of IL on the morphology and function of guinea pig peritoneal macrophages in vitro. Starch-induced macrophages were exposed to IL for up to 48 hours. Ingestion of increasing amounts of IL over the 48-hour period was confirmed by transmission electron microscopy and by oil red O stain. The uptake of the IL was associated with marked morphologic changes characterized by a decreased ability of the cells to spread and by a decrease in the number and degree of complexity of the membrane ruffles. The ingestion of IL also resulted in decreased capacity of the cells to associate with latex beads (5.7 mu in diameter) or Candida albicans and decreased capacity to adhere to and ingest sheep erythrocytes coated with IgG. After ingestion of latex beads 0.46 mu in diameter, which are similar in size to IL particles, macrophages had normal morphology and function, indicating that neither the morphologic nor functional abnormalities were due to a nonspecific effect of ingestion of small particles. Alterations of human reticuloendothelial macrophage function similar to the effects observed here could compromise host defense against infection.
对接受过静脉注射脂肪乳剂（IL）的人体研究表明，网状内皮吞噬细胞中存在脂肪色素和脂肪滴。临床数据和体外研究表明，这些细胞功能不正常。我们已经在体外研究了IL对豚鼠腹腔巨噬细胞形态和功能的影响。用淀粉诱导产生的巨噬细胞暴露于IL中长达48小时。通过透射电子显微镜和油红O染色证实，在48小时内巨噬细胞摄取了越来越多的IL。IL的摄取与明显的形态学变化有关，其特征是细胞铺展能力下降以及膜皱褶的数量和复杂程度降低。IL的摄取还导致细胞与乳胶珠（直径5.7μm）或白色念珠菌结合的能力下降，以及与包被有IgG的绵羊红细胞粘附和摄取的能力下降。摄取直径0.46μm的乳胶珠后，巨噬细胞具有正常的形态和功能，乳胶珠大小与IL颗粒相似，这表明形态学和功能异常均不是由于摄取小颗粒的非特异性作用所致。与这里观察到的效应类似的人类网状内皮巨噬细胞功能改变可能会损害宿主对感染的防御能力。
