Stern C D
Anat Embryol (Berl). 1979 Jul 26;156(3):319-29. doi: 10.1007/BF00299630.
As a result of extensive mitotic index analysis in colchicine-arrested chick embryos during gastrulation, it was ascertained that the primitive streak is a region of elevated mitotic index as compared to the surrounding tissue. Along the cephalo-caudal axis, the embryo displays two large peaks of mitotic index, one at the posterior end of the primitive streak and the other just anterior to Hensen's node. The length of the various phases of the mitotic period was determined in vitro by time-lapse filming, and the colchicine-arrested mitotic indices in vivo and in vitro were determined and compared for various regions. Some observations regarding the orientation of mitotic spindles and abnormal mitosis in vitro are also included, and the relevance of the above observations to early embryonic development is discussed.
通过对处于原肠胚形成期的秋水仙碱处理的鸡胚进行广泛的有丝分裂指数分析，确定与周围组织相比，原条是有丝分裂指数升高的区域。沿着头-尾轴，胚胎显示出两个有丝分裂指数的大峰值，一个在原条的后端，另一个就在亨氏结前方。通过延时拍摄在体外确定了有丝分裂期各个阶段的时长，并对体内和体外不同区域的秋水仙碱处理的有丝分裂指数进行了测定和比较。还包括了一些关于体外有丝分裂纺锤体方向和异常有丝分裂的观察结果，并讨论了上述观察结果与早期胚胎发育的相关性。
