Bjerknes M, Cheng H
Department of Anatomy, University of Toronto, Ontario, Canada.
Biophys J. 1989 May;55(5):1011-5. doi: 10.1016/S0006-3495(89)82899-1.
The three-dimensional orientation of mitoses in mouse small intestinal crypts of Lieberkuhn was determined from multiple projections of the mitotic figures in whole mounts of isolated intestinal crypts. We found evidence of a significant orientational bias for mitoses whose daughter cells would be added along the long axis of the crypt, and thus conform to the maintenance of the cylindrical shape of the intestinal crypt. However, we also observed many mitoses whose progeny must be rearranged if the simple cylindrical shape of the intestinal crypt is to be maintained. Our results indicate that the ultimate behavior of progeny cells and hence of local tissue form may not strictly depend on the orientation of mitosis. The methods presented may also be used in the study of mitotic orientation in other tissues.
通过对分离的小肠隐窝整装片中有丝分裂图像的多个投影，确定了小鼠利伯kühn小肠隐窝中有丝分裂的三维取向。我们发现，对于那些子细胞将沿隐窝长轴添加从而符合小肠隐窝圆柱形形态维持的有丝分裂，存在明显的取向偏差证据。然而，我们也观察到许多有丝分裂，如果要维持小肠隐窝简单的圆柱形形态，其子代细胞必须重新排列。我们的结果表明，子代细胞的最终行为以及局部组织形态可能并不严格取决于有丝分裂的取向。所介绍的方法也可用于研究其他组织中的有丝分裂取向。
