Sheridan P J, Herbert D C
Anat Rec. 1979 Aug;194(4):507-10. doi: 10.1002/ar.1091940404.
Two-day-old female rats were injected with 5 nmole/kg of 6,7-3H-11 beta-methoxy-17-ethylestradiol (R 2858 = moxestrol) and killed one hour later. The animals were decapitated and, the pituitary glands were removed, mounted on tissue holders and frozen in liquified propane. The tissue was then processed for autoradiography according to the thaw-mount technique. At the end of the exposure time, prior to photographic development, some of the tissue was fixed in 10% formalin and then photographically developed for autoradiography. The fixed tissue was subsequently stained immunocytochemically using antibodies to luteinizing hormone or prolactin. Between 10 and 15% of the cells of the pars distalis concentrated the synthetic estrogen or its metabolite. The immunocytochemical procedure revealed that both LH-gonadotrophs and lactotrophs concentrated the steroid. These studies along with earlier studies suggest that the neonatal rat pituitary contains only a small portion of the adult complement of estrogen receptors and that these receptors are dispersed across a number of cell types.
给两日龄雌性大鼠注射5纳摩尔/千克的6,7-³H-11β-甲氧基-17-乙基雌二醇（R 2858 = 莫昔司特），一小时后处死。将动物断头，取出垂体，固定在组织架上并在液化丙烷中冷冻。然后根据解冻贴片技术对组织进行放射自显影处理。在曝光时间结束后，在进行显影之前，将部分组织固定在10%福尔马林中，然后进行显影以进行放射自显影。随后使用促黄体生成素或催乳素抗体对固定组织进行免疫细胞化学染色。远侧部10%至15%的细胞浓缩了合成雌激素或其代谢产物。免疫细胞化学方法显示促黄体生成素促性腺激素细胞和催乳激素细胞都浓缩了该类固醇。这些研究以及早期研究表明，新生大鼠垂体仅含有成年大鼠雌激素受体总量的一小部分，并且这些受体分散在多种细胞类型中。
