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吸附色谱法在晚期妊娠尿雌激素比色测定中的应用(作者译)

[Application of adsorption chromatography to the colorimetric determination of urinary estrogens in late pregnancy (author's transl)].

作者信息

Yapo A E, Barthélémy-Clavey V, Racadot A, Mizon J

出版信息

Ann Biol Clin (Paris). 1979;37(2):107-11.

PMID:475071
Abstract

A new extraction procedure of urinary estrogens, based upon adsorption chromatography on Amberlite XAD-2, was deviced for a selective and complete extraction of these components. Estrogens were then quantified, without preliminar hydrolysis, by the colorimetric Kober reaction, modified by Ittrich. The reliability of this method has been established by comparison with four other methods.

摘要

基于Amberlite XAD - 2吸附色谱法,设计了一种新的尿雌激素提取方法,用于选择性且完全地提取这些成分。然后,雌激素在不进行预水解的情况下,通过伊特里希改良的比色柯柏反应进行定量。通过与其他四种方法比较,确立了该方法的可靠性。

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