Kuehne R W
Appl Microbiol. 1973 Sep;26(3):239-43. doi: 10.1128/am.26.3.239-243.1973.
To effectively characterize newly recognized viruses (Marburg, Lassa, etc.) and to study other highly virulent infections for which no effective prophylaxis or therapy exists, special containment facilities must be utilized and conventional techniques modified to minimize risk to laboratory personnel. This paper describes a laboratory suite for such studies, contained within a larger research facility; two separate biological safety cabinet systems, animal rooms support laboratories, change room facilities, shower, air lock, and other safety features are contained in the area. Details of design, construction, airflows, and equipment are described in addition to a discussion of operation, techniques, and modification of laboratory equipment utilized in actual studies.
为有效鉴定新发现的病毒（如马尔堡病毒、拉沙病毒等），并研究尚无有效预防或治疗方法的其他高致病性感染，必须使用特殊的防护设施，并对常规技术进行改进，以将对实验室人员的风险降至最低。本文介绍了一套用于此类研究的实验室套件，它位于一个更大的研究设施内；该区域包含两个独立的生物安全柜系统、动物房辅助实验室、更衣室设施、淋浴间、气闸以及其他安全设施。除了讨论实际研究中使用的操作、技术和实验室设备的改装外，还介绍了设计、建造、气流和设备的详细情况。
