嗜热芽孢杆菌脂肪酸组成的营养改变
Nutritional alteration of the fatty acid composition of a thermophilic Bacillus species.
作者信息
Daron H H
出版信息
J Bacteriol. 1973 Dec;116(3):1096-9. doi: 10.1128/jb.116.3.1096-1099.1973.
The fatty acid composition of a thermophilic Bacillus sp. was altered by the addition of isobutyrate, isovalerate, alpha-methylbutyrate, leucine, and isoleucine to the growth medium. With isobutyrate, 81% of the fatty acids had 16 carbon atoms and 79% were iso-fatty acids with an even number of carbon atoms. With leucine, 58% of the fatty acids had 15 carbon atoms and 86% were iso-fatty acids with an odd number of carbon atoms. With isoleucine, 72% of the fatty acids had 17 carbon atoms and 88% were anteiso-fatty acids with an odd number of carbon atoms. Thus, by altering the composition of the growth medium, cells were produced in which the majority of the fatty acids had either 15, 16, or 17 carbons and belonged to each of the three groups of branched-chain fatty acids. The wide variation observed in the fatty acid composition makes it unlikely that any specific branched-chain fatty acid is required for vital functions.
嗜热芽孢杆菌的脂肪酸组成通过在生长培养基中添加异丁酸、异戊酸、α-甲基丁酸、亮氨酸和异亮氨酸而发生改变。添加异丁酸时，81%的脂肪酸含有16个碳原子，79%是具有偶数碳原子的异脂肪酸。添加亮氨酸时，58%的脂肪酸含有15个碳原子，86%是具有奇数碳原子的异脂肪酸。添加异亮氨酸时，72%的脂肪酸含有17个碳原子，88%是具有奇数碳原子的前异脂肪酸。因此，通过改变生长培养基的组成，可产生细胞，其中大多数脂肪酸含有15、16或17个碳，并属于三类支链脂肪酸中的每一类。在脂肪酸组成中观察到的广泛变化使得不太可能任何特定的支链脂肪酸对于生命功能都是必需的。
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