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嗜热芽孢杆菌属中异柠檬酸裂解酶的存在

Occurrence of isocitrate lyase in a thermophilic bacillus species.

作者信息

Daron H H

出版信息

J Bacteriol. 1967 Feb;93(2):703-10. doi: 10.1128/jb.93.2.703-710.1967.

DOI:10.1128/jb.93.2.703-710.1967
PMID:6020570
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC276498/
Abstract

A thermophilic, sporeforming bacterium has been isolated from soil on a medium containing acetate as a carbon source. This organism is similar to Bacillus stearothermophilus in most respects but differs in its inability to hydrolyze starch. Isocitrate lyase is present in cell-free extracts of organisms grown in a medium with acetate as a carbon source. The specific activity was 400 times lower in extracts of organisms utilizing glucose as a carbon source. With crude extracts, enzyme activity was strongly stimulated by Mg(++), but cysteine and ethylenediaminetetraacetate had little effect. It appeared to be more heat-stable than the pure isocitrate lyase from Pseudomonas indigofera.

摘要

从含有乙酸盐作为碳源的培养基中的土壤中分离出一种嗜热、产芽孢的细菌。这种微生物在大多数方面与嗜热脂肪芽孢杆菌相似，但在其不能水解淀粉方面有所不同。异柠檬酸裂解酶存在于以乙酸盐作为碳源的培养基中生长的微生物的无细胞提取物中。在利用葡萄糖作为碳源的微生物提取物中，比活性低400倍。对于粗提取物，酶活性受到Mg(++)的强烈刺激，但半胱氨酸和乙二胺四乙酸的影响很小。它似乎比来自产靛假单胞菌的纯异柠檬酸裂解酶更耐热。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66f4/276498/2c7315e5663a/jbacter00408-0237-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66f4/276498/d19a0fbdad79/jbacter00408-0235-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66f4/276498/129d665e7802/jbacter00408-0236-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66f4/276498/2c7315e5663a/jbacter00408-0237-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66f4/276498/d19a0fbdad79/jbacter00408-0235-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66f4/276498/129d665e7802/jbacter00408-0236-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/66f4/276498/2c7315e5663a/jbacter00408-0237-a.jpg

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Occurrence of isocitrate lyase in a thermophilic bacillus species.嗜热芽孢杆菌属中异柠檬酸裂解酶的存在
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引用本文的文献

1
A polyphasic taxonomic study of thermophilic bacilli from a wide geographical area.对来自广泛地理区域的嗜热芽孢杆菌进行的多相分类研究。
Antonie Van Leeuwenhoek. 1993;64(3-4):357-86. doi: 10.1007/BF00873093.
2
Prototrophic thermophilic bacillus: isolation, properties, and kinetics of growth.原养型嗜热芽孢杆菌：分离、特性及生长动力学
J Bacteriol. 1969 Aug;99(2):414-7. doi: 10.1128/jb.99.2.414-417.1969.
3
Fatty acid composition of lipid extracts of a thermophilic Bacillus species.一种嗜热芽孢杆菌属物种脂质提取物的脂肪酸组成。

本文引用的文献

1
Thermal enzymes.热稳定酶
Arch Biochem. 1949 Nov;24(1):75-82.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
Thermostable alpha-amylase of Bacillus stearothermophilus. I. Crystallization and some general properties.嗜热脂肪芽孢杆菌的耐热α-淀粉酶。I. 结晶及一些一般性质。
J Bacteriol. 1970 Jan;101(1):145-51. doi: 10.1128/jb.101.1.145-151.1970.
4
Alternate pathways of metabolism of short-chain fatty acids.短链脂肪酸的替代代谢途径。
Bacteriol Rev. 1968 Mar;32(1):1-26. doi: 10.1128/br.32.1.1-26.1968.
5
Nutritional alteration of the fatty acid composition of a thermophilic Bacillus species.嗜热芽孢杆菌脂肪酸组成的营养改变
J Bacteriol. 1973 Dec;116(3):1096-9. doi: 10.1128/jb.116.3.1096-1099.1973.
6
Isocitrate lyase from a thermophilic Bacillus: effect of salts on enzyme activity.嗜热芽孢杆菌中的异柠檬酸裂解酶：盐对酶活性的影响。
J Bacteriol. 1973 Dec;116(3):1160-9. doi: 10.1128/jb.116.3.1160-1169.1973.
7
Isolation and characterization of isocitrate lyase from a thermophilic Bacillus sp.嗜热芽孢杆菌中异柠檬酸裂解酶的分离与鉴定
Biochem J. 1978 Jul 1;173(1):165-77. doi: 10.1042/bj1730165.
J Biol Chem. 1961 Nov;236:2952-7.
4
The influence of growth substrates on metabolic pathways in Micrococcus denitrificans.生长底物对反硝化微球菌代谢途径的影响。
Biochim Biophys Acta. 1960 Mar 25;39:9-24. doi: 10.1016/0006-3002(60)90117-7.
5
Dissimilation of citric acid by extracts of Penicillium chrysogenum.产黄青霉提取物对柠檬酸的异化作用
Arch Biochem Biophys. 1960 Jan;86:102-9. doi: 10.1016/0003-9861(60)90376-3.
6
Distribution and formation of isocitritase.异柠檬酸裂合酶的分布与形成
Nature. 1955 Apr 30;175(4461):774-5. doi: 10.1038/175774b0.
7
ISOCITRATE LYASE FROM PSEUDOMONAS INDIGOFERA. II. ACTIVATION, INHIBITION AND STABILIZATION.
Biochim Biophys Acta. 1965 Jan;96:123-33. doi: 10.1016/0005-2787(65)90616-7.
8
ISOCITRATE LYASE FROM PSEUDOMONAS INDIGOFERA. I. PREPARATION, AMINO ACID COMPOSITION AND MOLECULAR WEIGHT.来自产靛假单胞菌的异柠檬酸裂合酶。I. 制备、氨基酸组成及分子量
Biochim Biophys Acta. 1965 Jan;96:114-22. doi: 10.1016/0005-2787(65)90615-5.
9
Activation of isocitrate lyase and triosephosphate dehydrogenase in Azotobacter vinelandii extracts.维涅兰德固氮菌提取物中异柠檬酸裂解酶和磷酸丙糖脱氢酶的激活
Biochim Biophys Acta. 1963 Feb 12;67:226-39. doi: 10.1016/0006-3002(63)91820-1.
10
On the mechanism of anaerobic formation of succinate by Proteus vulgaris.关于普通变形杆菌厌氧生成琥珀酸的机制。
J Biochem. 1961 May;49:421-6. doi: 10.1093/oxfordjournals.jbchem.a127320.