De Loecker W, De Wever F, Leyman A M, Doms D
Arch Int Pharmacodyn Ther. 1979 Apr;238(2):333-43.
The effects of cortisol on protein metabolism were examined in rhabdomyosarcoma tissue, experimentally provoked in rats by a single intramuscular injection of cobalt powder into the adductor muscle of the hind limb. In vivo treatment of tumour bearing animals with 1 and 10 mg cortisol resulted in a reduced incorporation of [2-14C] glycine into the tumour proteins. In vitro application of 0.01 to 100 micrograms cortisol on tumour slices equally reduced the amino acid incorporation into the proteins. These inhibitions could not be explained by modifications in protein, RNA, DNA content or by changes in the membrane function. The factors inhibiting the amino acid incorporation into tumour proteins could be located in the 105,000 X g supernatant protein fraction. The tumour transfer RNA methylase activity became markedly inhibited by cortisol treatment.
通过向后肢内收肌单次肌肉注射钴粉在大鼠中诱发横纹肌肉瘤组织，研究了皮质醇对蛋白质代谢的影响。用1毫克和10毫克皮质醇对荷瘤动物进行体内治疗，导致[2-¹⁴C]甘氨酸掺入肿瘤蛋白的量减少。在肿瘤切片上体外应用0.01至100微克皮质醇同样减少了氨基酸掺入蛋白质的量。这些抑制作用无法通过蛋白质、RNA、DNA含量的改变或膜功能的变化来解释。抑制氨基酸掺入肿瘤蛋白的因素可能位于105,000×g上清蛋白组分中。皮质醇处理显著抑制了肿瘤转移RNA甲基化酶活性。
