Bouchon B, Portoukalian J, Bornet H
Biochim Biophys Acta. 1985 Aug 22;836(1):143-52. doi: 10.1016/0005-2760(85)90230-9.
The glycosphingolipids of human thyroid were isolated and characterized by gas-liquid chromatography and sequential enzymic hydrolysis. The major purified components were identified as glucosyl- and galactosyl-ceramides, lactosyl- and galabiosylceramides, globotriaosyl- and globotetraosylceramides. The long-chain base analyses showed a high proportion of phytosphingosine in glycosylceramide and galabiosylceramide. Fatty acids in 22:0, 24:0, 24:1 prevailed, especially in the cerebroside fraction, with a significant content of alpha-hydroxylated species in galactosylceramide. Female thyroid had a very low content of galabiosylceramide and a higher content of glucosylceramide, as compared to male. No significant difference was found in the other neutral glycosphingolipids and gangliosides.
通过气液色谱法和顺序酶促水解法对人甲状腺中的糖鞘脂进行了分离和表征。主要纯化成分被鉴定为葡糖基神经酰胺和半乳糖基神经酰胺、乳糖基神经酰胺和半乳糖二糖基神经酰胺、球三糖基神经酰胺和球四糖基神经酰胺。长链碱分析表明，糖基神经酰胺和半乳糖二糖基神经酰胺中植物鞘氨醇的比例很高。以22:0、24:0、24:1为主的脂肪酸，尤其是在脑苷脂部分，半乳糖基神经酰胺中α-羟基化物种的含量显著。与男性相比，女性甲状腺中半乳糖二糖基神经酰胺含量非常低，葡糖基神经酰胺含量较高。在其他中性糖鞘脂和神经节苷脂中未发现显著差异。
