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半乳糖脑苷脂在金黄仓鼠肾脏、肝脏和肺中的免疫组织化学定位

Immunohistochemical localization of galactocerebroside in kidney, liver, and lung of golden hamster.

作者信息

Sakakibara K, Iwamori M, Uchida T, Nagai Y

出版信息

Experientia. 1981 Jul 15;37(7):712-4. doi: 10.1007/BF01967938.

Abstract

Localization of galactocerebroside in kidney, liver, and lung of hamster was studied by the immunoperoxidase method using an affinity-purified specific antibody. Epithelial cells of the following anatomical sites were labelled with the antibody: distal tubuli, ascending limbs of Henle's loops, and collecting tubuli in kidney; periportal bile ducts and hepatic parenchyma in liver; bronchioli and alveoli in lung. The existence of galactocerebroside in these 3 organs was also confirmed by chemical analysis.

摘要

采用亲和纯化的特异性抗体,通过免疫过氧化物酶法研究了半乳糖脑苷脂在仓鼠肾脏、肝脏和肺中的定位。以下解剖部位的上皮细胞被该抗体标记:肾脏中的远曲小管、髓袢升支和集合小管;肝脏中的门周胆管和肝实质;肺中的细支气管和肺泡。通过化学分析也证实了这3种器官中存在半乳糖脑苷脂。

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