Jones L M, Diaz R, Taylor A G
Br J Exp Pathol. 1973 Oct;54(5):492-508.
Protein allergens were prepared from smooth strain Rev. 1 and rough strain B115 using a cold saline method of extraction. Guineapig skin tests showed the two preparations to be of similar potency and specificity. Gel filtration on Sephadex G-75 showed that the skin test reactive fractions of each preparation were composed of a mixture of proteins with molecular weights in the range of 12,000 to 50,000. Polyacrylamide gel electrophoresis and immunoelectrophoresis indicated that the preparations consisted of at least 12 similar, if not identical, protein constituents. At least 20 protein bands were revealed with polyacrylamide gel isoelectric focusing, which appeared to be almost identical in the two preparations. Smooth lipopolysaccharide antigen, tested in normal and infected guineapigs, was of a lower potency than protein allergens and the eyrthematous response occurred earlier, resembling an antibody mediated reaction rather than delayed hypersensitivity. The allergen prepared from the rough strain contained no detectable smooth lipopolysaccharide antigen and therefore did not stimulate a secondary rise in antibody to smooth cells in sensitized animals. As the presence of smooth lipopolysaccharide antigen in an allergen appears to be unnecessary for provoking delayed hypersensitivity responses, and undesirable because it may stimulate the production of antibodies which interfere with diagnosis, it is preferable to prepare allergens from rough strains of brucella.
采用冷盐水提取法从光滑型Rev. 1菌株和粗糙型B115菌株制备蛋白质过敏原。豚鼠皮肤试验表明，这两种制剂的效力和特异性相似。在Sephadex G - 75上进行凝胶过滤显示，每种制剂的皮肤试验反应性组分由分子量在12,000至50,000范围内的蛋白质混合物组成。聚丙烯酰胺凝胶电泳和免疫电泳表明，这些制剂至少由12种相似（即便不是相同）的蛋白质成分组成。聚丙烯酰胺凝胶等电聚焦显示至少有20条蛋白带，这两种制剂中的蛋白带几乎相同。在正常和感染的豚鼠中测试的光滑型脂多糖抗原，其效力低于蛋白质过敏原，且红斑反应出现得更早，类似于抗体介导的反应而非迟发型超敏反应。从粗糙型菌株制备的过敏原不含可检测到的光滑型脂多糖抗原，因此不会刺激致敏动物体内针对光滑型细胞的抗体再次升高。由于过敏原中光滑型脂多糖抗原的存在对于引发迟发型超敏反应似乎并非必要，而且因为它可能刺激产生干扰诊断的抗体而不受欢迎，所以最好从布鲁氏菌粗糙型菌株制备过敏原。
