Major L F, Lerner P, Ballenger J C, Brown G L, Goodwin F K, Lovenberg W
Biol Psychiatry. 1979 Apr;14(2):337-44.
Cerebrospinal fluid (CSF) dopamine-beta-hydroxylase (DBH) activity in 32 male alcoholics was measured using a modification of the radioenzymatic method of Molinoff et al. In most, the CSF was obtained before treatment with disulfiram, while in others it was obtained while they were on the drug (250 or 500 mg). As expected, treatment with this reversible DBH inhibitor had no effect on the activity of the enzyme measured in our in vitro assay. However, low pretreatment DBH activity was found to correlate with adverse reactions to disulfiram. Mean DBH activity of four individuals who went on to become psychotic on disulfiram was 0.13 +/- 0.02 nmole/ml per hr (mean +/- SEM). An additional four individuals who developed dysphoric but nonpsychotic reactions had a mean DBH of 0.23 +/- 0.03. Both these values were significantly lower than the mean DBH activity of the remaining 24 individuals treated with disulfiram who had no adverse side effects, 0.53 +/- 0.06 p less than 0.02 and p less than 0.05, respectively, 2-tailed t-test.
采用对莫利诺夫等人的放射酶法的改进方法，测定了32名男性酗酒者脑脊液（CSF）中的多巴胺-β-羟化酶（DBH）活性。大多数情况下，脑脊液是在使用双硫仑治疗前采集的，而在其他情况下，脑脊液是在他们服用该药物（250或500毫克）时采集的。正如预期的那样，使用这种可逆的DBH抑制剂进行治疗，对我们体外测定的酶活性没有影响。然而，发现治疗前DBH活性较低与对双硫仑的不良反应相关。在双硫仑治疗期间出现精神病症状的4名个体的平均DBH活性为每小时0.13±0.02纳摩尔/毫升（平均值±标准误）。另外4名出现烦躁但无精神病反应的个体的平均DBH为0.23±0.03。这两个值均显著低于其余24名接受双硫仑治疗且无不良副作用的个体的平均DBH活性，分别为0.53±0.06，双侧t检验，p值均小于0.02和0.05。
