Powers K H, Ebert M H
Biomed Mass Spectrom. 1979 May;6(5):187-90. doi: 10.1002/bms.1200060503.
Quantification of low levels of psychotropic drugs (10(-7) to 10(-9) g ml-1) in small volumes of plasma requires sensitive and accurate methods. Validation of these methods is best achieved by comparing results obtained using several techniques. In this study, amphetamine levels in plasma were measured using gas chromatography mass spectrometry and radioimmunoassay. Correlation of the results obtained by the two methods was found to be positive and high (R = 0.9822). The average coefficient of variation between assays for gas chromatography mass spectrometry was 5.8% and for radioimmunoassay was 12.3%, while the average coefficient of variation within assays for gas chromatography mass spectrometry was 4.9% and for radioimmunoassay 6.9%. Although gas chromatography mass spectrometry was 1.9 times more sensitive than radioimmunoassay, for most purposes, the convenience of the radioimmunoassay method outweights the technical superiority of gas chromatography mass spectrometry.
对小体积血浆中低水平精神药物（10⁻⁷至10⁻⁹克/毫升）进行定量需要灵敏且准确的方法。这些方法的验证最好通过比较使用多种技术获得的结果来实现。在本研究中，使用气相色谱 - 质谱联用仪和放射免疫分析法测定血浆中的苯丙胺水平。发现两种方法所得结果的相关性为正且较高（R = 0.9822）。气相色谱 - 质谱联用仪测定的平均批间变异系数为5.8%，放射免疫分析法为12.3%，而气相色谱 - 质谱联用仪测定的平均批内变异系数为4.9%，放射免疫分析法为6.9%。尽管气相色谱 - 质谱联用仪的灵敏度比放射免疫分析法高1.9倍，但对于大多数目的而言，放射免疫分析法的便利性超过了气相色谱 - 质谱联用仪的技术优势。
