Sussenbach J S, Ellens D J, Jansz H S
J Virol. 1973 Nov;12(5):1131-8. doi: 10.1128/JVI.12.5.1131-1138.1973.
Replicative intermediates of adenovirus type 5 DNA contain large stretches of single-stranded DNA. We have shown that this single-stranded DNA is mainly of parental origin, whereas all new DNA synthesized during one round of replication has a double-stranded structure. Hybridization experiments of the single-stranded DNA with isolated complementary strands of adenovirus type 5 DNA showed that this DNA hybridized only with the viral L-strand (the strand with the lower equilibrium density in alkaline CsCl) indicating that it represents the viral H-strand. This observation implies that replication always starts from one and the same molecular end. Electron microscopy of partially denatured Y-shaped intermediates confirmed this and showed that replication started from the molecular right end (the end richest in A-T base pairs). In conclusion, we have shown that replication of adenovirus type 5 DNA starts at the molecular right end, displacing the parental H-strand.
5型腺病毒DNA的复制中间体含有大片段单链DNA。我们已经表明，这种单链DNA主要来自亲本，而在一轮复制过程中合成的所有新DNA都具有双链结构。单链DNA与分离出的5型腺病毒DNA互补链的杂交实验表明，这种DNA仅与病毒L链（在碱性CsCl中平衡密度较低的链）杂交，这表明它代表病毒H链。这一观察结果意味着复制总是从同一个分子末端开始。部分变性的Y形中间体的电子显微镜观察证实了这一点，并表明复制从分子右端（富含A-T碱基对的末端）开始。总之，我们已经表明，5型腺病毒DNA的复制从分子右端开始，取代亲本H链。
