Steinetz B G, Matthews J R, Butler M C, Thompson S W
Am J Pathol. 1973 Dec;73(3):735-46.
A specific local erosion of the medial edges of the pubic bones is induced by administration of estradiol cyclopentylpropionate (ECP) to thyroparathyroidectomized mice. This paper presents both histochemical and biochemical evidence that porcine thyrocalcitonin (TCT) inhibits this estrogen-mediated resorption of the pubic symphysis. A marked increase in osteoclasts with a resultant increase in demonstrable sites of acid phosphatase (AcP) activity was observed in serial sections of resorbing pubic symphyses from ECP-treated animals. Bioassay of excised pubic symphyses revealed a concomitant increase in AcP activity. When mice were treated with TCT in combination with ECP, a marked decrease in osteoclasts with a resultant decrease in demonstrable sites of AcP activity and inhibition of the resorption process occurred. The mouse pubic symphysis thus appears to offer a model for exploring cellular mechanisms by which TCT regulates bone metabolism.
给甲状旁腺切除的小鼠注射环戊丙酸雌二醇（ECP）会导致耻骨内缘出现特定的局部侵蚀。本文提供了组织化学和生物化学证据，表明猪甲状腺降钙素（TCT）可抑制这种雌激素介导的耻骨联合吸收。在接受ECP治疗的动物耻骨联合吸收的连续切片中，观察到破骨细胞显著增加，同时酸性磷酸酶（AcP）活性的可显示位点也随之增加。对切除的耻骨联合进行生物测定，发现AcP活性也相应增加。当小鼠同时接受TCT和ECP治疗时，破骨细胞显著减少，AcP活性的可显示位点随之减少，吸收过程受到抑制。因此，小鼠耻骨联合似乎为探索TCT调节骨代谢的细胞机制提供了一个模型。
