Cameron I L, Pool M R, Hoage T R
Cell Tissue Kinet. 1979 Jul;12(4):445-51. doi: 10.1111/j.1365-2184.1979.tb00167.x.
Adult mice were pulse labeled with tritiated thymidine [3H]TdR and killed 9 hr later. A low level incorporation of [3H]TdT into the nuclear DNA of Purkinje neurons was found in autoradiographs. Enzymatic digestions with DNase and with RNase in combination with autoradiographic grain counts indicate that a portion of nuclear DNA is not stable in the Purkinje nucleus. These results are discussed in light of reports of the stable nature of DNA in Purkinje neurons of adult mice.
成年小鼠用氚标记的胸腺嘧啶核苷[3H]TdR进行脉冲标记，并在9小时后处死。在放射自显影片中发现[3H]TdT低水平掺入浦肯野神经元的核DNA中。用DNA酶和RNA酶进行酶消化并结合放射自显影颗粒计数表明，浦肯野核中的一部分核DNA不稳定。根据成年小鼠浦肯野神经元中DNA稳定性的报道对这些结果进行了讨论。
