Frère J M, Ghuysen J M, Perkins H R, Nieto M
Biochem J. 1973 Nov;135(3):463-8. doi: 10.1042/bj1350463.
A procedure allowing the purification of milligram amounts of the exocellular dd-carboxypeptidase-transpeptidase from Streptomyces R61 to protein homogeneity (95% purity) is described. The isolated protein has a molecular weight of about 38000 and consists of one polypeptide chain. Its amino acid composition is presented.
本文描述了一种从链霉菌R61中纯化毫克量胞外dd-羧肽酶-转肽酶至蛋白质均一性(纯度95%)的方法。分离得到的蛋白质分子量约为38000,由一条多肽链组成。文中给出了其氨基酸组成。