链霉菌DD-羧肽酶的肽类抑制剂。
Peptide inhibitors of Streptomyces DD-carboxypeptidases.
作者信息
Nieto M, Perkins H R, Leyh-Bouille M, Frère J M, Ghuysen J M
出版信息
Biochem J. 1973 Jan;131(1):163-71. doi: 10.1042/bj1310163.
Abstract
- Peptides that inhibit the dd-carboxypeptidases from Streptomyces strains albus G and R61 were synthesized. They are close analogues of the substrates of these enzymes. The enzymes from albus G and R61 strains are in general inhibited by the same peptides, but the enzyme from strain R39 differs considerably. 2. The two C-terminal residues of the peptide substrates and inhibitors appear to be mainly responsible for the initial binding of the substrate to the enzymes from albus G and R61 strains. The side chain in the third residue from the C-terminus seems critical in inducing catalytic activity. 3. Experimental evidence is presented suggesting that the amide bond linking the two C-terminal residues has a cis configuration when bound to the enzymes from strains albus G and R61. 4. The peptide inhibitors are not antibiotics against the same micro-organisms.
摘要
- 合成了抑制来自白色链霉菌G菌株和R61菌株的双功能羧肽酶的肽。它们是这些酶底物的类似物。来自白色链霉菌G菌株和R61菌株的酶通常被相同的肽抑制，但来自R39菌株的酶有很大差异。2. 肽底物和抑制剂的两个C末端残基似乎主要负责底物与来自白色链霉菌G菌株和R61菌株的酶的初始结合。C末端第三个残基的侧链在诱导催化活性方面似乎至关重要。3. 实验证据表明，连接两个C末端残基的酰胺键在与来自白色链霉菌G菌株和R61菌株的酶结合时具有顺式构型。4. 肽抑制剂对相同的微生物不是抗生素。
相似文献
1
Peptide inhibitors of Streptomyces DD-carboxypeptidases.链霉菌DD-羧肽酶的肽类抑制剂。
Biochem J. 1973 Jan;131(1):163-71. doi: 10.1042/bj1310163.
2
Streptomyces DD-carboxypeptidases as transpeptidases. The specificity for amino compounds acting as carboxyl acceptors.作为转肽酶的链霉菌DD-羧肽酶。对充当羧基受体的氨基化合物的特异性。
Biochem J. 1973 Apr;131(4):707-18. doi: 10.1042/bj1310707.
3
DD-carboxypeptidase-transpeptidase and killing site of beta-lactam antibiotics in Streptomyces strains R39, R61, and K11.链霉菌菌株R39、R61和K11中的DD-羧肽酶-转肽酶及β-内酰胺抗生素的杀伤位点
Antimicrob Agents Chemother. 1973 Feb;3(2):181-7. doi: 10.1128/AAC.3.2.181.
4
Mode of interaction between beta-lactam antibiotics and the exocellular DD-carboxypeptidase--transpeptidase from Streptomyces R39.β-内酰胺抗生素与来自链霉菌R39的细胞外DD-羧肽酶-转肽酶之间的相互作用模式
Biochem J. 1976 Jun 1;155(3):623-9. doi: 10.1042/bj1550623.
5
Interaction between non-classical beta-lactam compounds and the Zn2+-containing G and serine R61 and R39 D-alanyl-D-alanine peptidases.非经典β-内酰胺化合物与含锌的G型和丝氨酸R61及R39 D-丙氨酰-D-丙氨酸肽酶之间的相互作用。
Biochem J. 1981 Oct 1;199(1):129-36. doi: 10.1042/bj1990129.
6
Transpeptidase activity of Streptomyces D-alanyl-D carboxypeptidases.
Proc Natl Acad Sci U S A. 1972 Mar;69(3):662-6. doi: 10.1073/pnas.69.3.662.
7
Studies on the primary structures of the exocellular D-alanyl-D-alanine peptidases of Streptomyces strain R61 and Actinomadura strain R39.
Biochim Biophys Acta. 1981 Dec 29;671(2):109-16. doi: 10.1016/0005-2795(81)90123-9.
8
Dipeptide binding to the extended active site of the Streptomyces R61 D-alanyl-D-alanine-peptidase: the path to a specific substrate.二肽与链霉菌R61 D-丙氨酰-D-丙氨酸肽酶延伸活性位点的结合：通向特异性底物之路
Biochemistry. 2000 Oct 10;39(40):12200-9. doi: 10.1021/bi001295w.
9
Binding of beta-lactam antibiotics to the exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R39.β-内酰胺抗生素与链霉菌R39的胞外D-羧肽酶-转肽酶的结合。
Biochem J. 1974 Oct;143(1):241-9. doi: 10.1042/bj1430241.
10
Structure of the wall peptidoglycan of Streptomyces R39 and the specificity profile of its exocellular DD-carboxypeptidase--transpeptidase for peptide acceptors.链霉菌R39细胞壁肽聚糖的结构及其胞外DD-羧肽酶-转肽酶对肽受体的特异性谱
Biochemistry. 1973 Mar 27;12(7):1243-51. doi: 10.1021/bi00731a001.
引用本文的文献
1
A peptidoglycan fragment triggers β-lactam resistance in Bacillus licheniformis.一个肽聚糖片段在地衣芽孢杆菌中触发了β-内酰胺类抗生素耐药性。
PLoS Pathog. 2012;8(3):e1002571. doi: 10.1371/journal.ppat.1002571. Epub 2012 Mar 15.
2
Specificity inversion of Ochrobactrum anthropi D-aminopeptidase to a D,D-carboxypeptidase with new penicillin binding activity by directed mutagenesis.通过定向诱变将嗜人苍白杆菌D-氨基肽酶特异性转化为具有新青霉素结合活性的D，D-羧肽酶。
Protein Sci. 2005 Sep;14(9):2296-303. doi: 10.1110/ps.051475305.
3
Fluorescence and circular dichroism studies on the Streptomyces R61 DD-carboxypeptidase-transpeptidase. Penicillin binding by the enzyme.链霉菌R61 D-羧肽酶-转肽酶的荧光和圆二色性研究。该酶与青霉素的结合。
Biochem J. 1973 Nov;135(3):493-505. doi: 10.1042/bj1350493.
4
Kinetics of concomitant transfer and hydrolysis reactions catalysed by the exocellular DD-carboxypeptidase-transpeptidase of streptomyces R61.链霉菌R61胞外DD-羧肽酶-转肽酶催化的伴随转移和水解反应的动力学
Biochem J. 1973 Nov;135(3):483-92. doi: 10.1042/bj1350483.
5
Molecular weight and amino acid composition of the exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R61.链霉菌R61胞外DD-羧肽酶-转肽酶的分子量及氨基酸组成
Biochem J. 1973 Nov;135(3):463-8. doi: 10.1042/bj1350463.
6
Streptomyces DD-carboxypeptidases as transpeptidases. The specificity for amino compounds acting as carboxyl acceptors.作为转肽酶的链霉菌DD-羧肽酶。对充当羧基受体的氨基化合物的特异性。
Biochem J. 1973 Apr;131(4):707-18. doi: 10.1042/bj1310707.
7
Interaction of penicillin with the bacterial cell: penicillin-binding proteins and penicillin-sensitive enzymes.青霉素与细菌细胞的相互作用：青霉素结合蛋白和青霉素敏感酶。
Bacteriol Rev. 1974 Sep;38(3):291-335. doi: 10.1128/br.38.3.291-335.1974.
8
Molecular weight, amino acid composition and physicochemical properties of the exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R39.链霉菌R39胞外DD-羧肽酶-转肽酶的分子量、氨基酸组成及理化性质
Biochem J. 1974 Oct;143(1):233-40. doi: 10.1042/bj1430233.
9
The synthesis of peptidoglycan in an autolysin-deficient mutant of Bacillus licheniformis N.C.T.C. 6346 and the effect of beta-lactam antibiotics, bacitracin and vancomycin.地衣芽孢杆菌N.C.T.C. 6346自溶素缺陷突变体中肽聚糖的合成以及β-内酰胺抗生素、杆菌肽和万古霉素的作用。
Biochem J. 1974 Jul;141(1):227-41. doi: 10.1042/bj1410227.
10
Membrane-bound DD-carboxypeptidases from Bacillus megaterium KM general properties, substrate specificity and sensitivity to penicillins, cephalosporins and peptide inhibitors of the activity at pH5.巨大芽孢杆菌KM的膜结合DD-羧肽酶：pH5条件下的一般性质、底物特异性以及对青霉素、头孢菌素和活性肽抑制剂的敏感性
Biochem J. 1974 Nov;143(2):391-402. doi: 10.1042/bj1430391.
本文引用的文献
1
Energetics of peptide formation.肽形成的能量学
Nature. 1952 May 31;169(4309):922. doi: 10.1038/169922a0.
2
CHEMICAL STRUCTURE OF BACTERIAL CELL WALLS. ORNITHINE AND 2,4-DIAMINOBUTYRIC ACID AS COMPONENTS OF THE CELL WALLS OF PLANT PATHOGENIC CORYNEBACTERIA.细菌细胞壁的化学结构。鸟氨酸和2,4-二氨基丁酸作为植物病原棒状杆菌细胞壁的成分
Nature. 1964 Mar 14;201:1105-7. doi: 10.1038/2011105a0.
3
Reaction of ninhydrin in acid solution with straight-chain amino acids containing two amino groups and its application to the estimation of alpha epsilon-diaminopimelic acid.茚三酮在酸性溶液中与含两个氨基的直链氨基酸的反应及其在α,ε-二氨基庚二酸测定中的应用。
Biochem J. 1957 Nov;67(3):416-23. doi: 10.1042/bj0670416.
4
2,6-Diamino-3-hydroxypimelic acid in microbial cell wall mucopeptide.微生物细胞壁粘肽中的2,6-二氨基-3-羟基庚二酸
Nature. 1965 Nov 27;208(5013):872-3. doi: 10.1038/208872a0.
5
Specificity of combination between mucopeptide precursors and vancomycin or ristocetin.粘肽前体与万古霉素或瑞斯托霉素之间结合的特异性。
Biochem J. 1969 Jan;111(2):195-205. doi: 10.1042/bj1110195.
6
Substrate requirements of the Streptomyces albus G DD carboxypeptidase.白色链霉菌G型DD羧肽酶的底物需求
Biochemistry. 1970 Jul 21;9(15):2961-70. doi: 10.1021/bi00817a005.
7
Mechanism of action of penicillins: a proposal based on their structural similarity to acyl-D-alanyl-D-alanine.青霉素的作用机制：基于其与酰基-D-丙氨酰-D-丙氨酸结构相似性的一种假说
Proc Natl Acad Sci U S A. 1965 Oct;54(4):1133-41. doi: 10.1073/pnas.54.4.1133.
8
Modifications of the acyl-D-alanyl-D-alanine terminus affecting complex-formation with vancomycin.影响与万古霉素形成复合物的酰基-D-丙氨酰-D-丙氨酸末端修饰
Biochem J. 1971 Aug;123(5):789-803. doi: 10.1042/bj1230789.
9
Penicillin-sensitive DD-carboxypeptidases from Streptomyces strains R39 and K11.来自链霉菌菌株R39和K11的青霉素敏感型DD-羧肽酶
Biochemistry. 1972 Mar 28;11(7):1290-8. doi: 10.1021/bi00757a027.
10
Penicillin-sensitive DD-carboxypeptidase from Streptomyces strain R 61.来自链霉菌菌株R 61的青霉素敏感型DD-羧肽酶
Biochemistry. 1971 May 25;10(11):2163-70. doi: 10.1021/bi00787a032.
Zotero 插件